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Our Abpromise guarantee covers the use of ab52017 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 44 kDa). The observed molecular weight corresponds to earlier findings in literature (Yasumoto et al , EMBO J. 2002 Jun 3;21(11):2703-14.; PMID: 12032083). Immunofluorescence: This antibody was successfully used to stain LEF1 in Zebrafish fish fins. Fins were fixed with paraformaldehyde at 4°C (See PMID 19015519).|
|EMSA||Use at an assay dependent concentration. PubMed: 22011226|
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