製品の概要

製品の特性

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アプリケーション

Our Abpromise guarantee covers the use of ab28316 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 46 kDa (predicted molecular weight: 44 kDa).

ターゲット情報

  • 機能Participates in the Wnt signaling pathway. Activates transcription of target genes in the presence of CTNNB1 and EP300. May play a role in hair cell differentiation and follicle morphogenesis. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by LEF1 and CTNNB1. Regulates T-cell receptor alpha enhancer function. Binds DNA in a sequence-specific manner. PIAG antagonizes both Wnt-dependent and Wnt-independent activation by LEF1 (By similarity). Isoform 3 lacks the CTNNB1 interaction domain and may be an antagonist for Wnt signaling. Isoform 5 transcriptionally activates the fibronectin promoter, binds to and represses transcription from the E-cadherin promoter in a CTNNB1-independent manner, and is involved in reducing cellular aggregation and increasing cell migration of pancreatic cancer cells. Isoform 1 transcriptionally activates MYC and CCND1 expression and enhances proliferation of pancreatic tumor cells.
  • 組織特異性Detected in thymus. Not detected in normal colon, but highly expressed in colon cancer biopsies and colon cancer cell lines. Expressed in several pancreatic tumors and weakly expressed in normal pancreatic tissue. Isoforms 1 and 5 are detected in several pancreatic cell lines.
  • 配列類似性Belongs to the TCF/LEF family.
    Contains 1 HMG box DNA-binding domain.
  • ドメインProline-rich and acidic regions are implicated in the activation functions of RNA polymerase II transcription factors.
  • 細胞内局在Nucleus. Found in nuclear bodies upon PIASG binding.
  • Information by UniProt
  • 参照データベース
  • 別名
    • DKFZp586H0919 antibody
    • FLJ46390 antibody
    • LEF 1 antibody
    • LEF-1 antibody
    • Lef1 antibody
    • LEF1_HUMAN antibody
    • Lymphoid enhancer binding factor 1 antibody
    • Lymphoid enhancer-binding factor 1 antibody
    • T cell specific transcription factor 1 alpha antibody
    • T cell-specific transcription factor 1-alpha antibody
    • TCF 1 alpha antibody
    • TCF1 alpha antibody
    • TCF1-alpha antibody
    • TCF10 antibody
    • TCF1alpha antibody
    • TCF7L3 antibody
    • Transcription factor T cell specific 1 alpha antibody
    see all

Anti-LEF1 antibody 画像

  • All lanes : Anti-LEF1 antibody (ab28316) at 5 µg/ml

    Lane 1 : Mock
    Lane 2 : TCF1
    Lane 3 : TCF4
    Lane 4 : Jurkat cell extract
    Lane 5 : Full length LEF1
    Lane 6 : Full length LEF1 Ø HMG
    Lane 7 : Recombinant full length LEF1 at 0.01 µg
    Lane 8 : LEF1 amino acids 1 - 192
    Lane 9 : LEF1 amino acids 1 - 276


    Predicted band size : 44 kDa

Anti-LEF1 antibody (ab28316) 使用論文

ab28316 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"