製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to LDOC1
  • アプリケーション
    適用あり: IHC-P, WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Rabbit, Horse, Guinea pig, Cow, Cat, Dog
  • 免疫原

    Synthetic peptide corresponding to a region within the N terminal amino acids 2-51 (VDELVLLLHA LLMRHRALSI ENSQLMEQLR LLVCERASLL RQVRPPSCPV) of human LDOC1 (NP_036449).

  • ポジティブ・コントロール
    • HepG2 whole cell lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab86126 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 17 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

ターゲット情報

  • 機能
    May have an important role in the development and/or progression of some cancers.
  • 組織特異性
    Ubiquitously expressed with high levels in brain ant thyroid and low expression in placenta, liver and leukocytes. Expressed as well in six of the seven human breast cancer cell lines examined.
  • 配列類似性
    Belongs to the LDOC1 family.
  • 細胞内局在
    Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • BCUR1 antibody
    • Breast cancer up regulated 1 antibody
    • Breast cancer upregulated 1 antibody
    • Gm366 antibody
    • Ldoc1 antibody
    • LDOC1_HUMAN antibody
    • Leucine zipper down regulated in cancer 1 antibody
    • Leucine zipper protein down regulated in cancer cells antibody
    • Leucine zipper protein down regulated in cancer cells antibody
    • Leucine zipper protein down-regulated in cancer cells antibody
    • Mar7 antibody
    • Mart7 antibody
    • Protein LDOC1 antibody
    • RGD1565644 antibody
    see all

画像



  • Predicted band size : 17 kDa
    Western blot analysis of Human fetal lung tissue lysate labeling LDOC1 with ab86126 at 1.0µg/ml.


  • Predicted band size : 17 kDa
    Western blot analysis of HeLa cell lysate labeling LDOC1 with ab86126 at 1.0µg/ml.
  • Anti-LDOC1 antibody (ab86126) at 1 µg/ml + HepG2 whole cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 17 kDa
    Observed band size : ~17 kDa (why is the actual band size different from the predicted?)
    Additional bands at : >60 kDa. We are unsure as to the identity of these extra bands.Gel concentration: 10-20%
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human thyroid tissue labelling LDOC1 with ab86126 at 5.0µg/ml.

参考文献

ab86126 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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