製品の概要

  • 製品名Anti-LDL Receptor antibody
    LDL Receptor 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to LDL Receptor
  • アプリケーション適用あり: Flow Cyt, IHC-Fr, IHC-P, WB, ICCmore details
  • 種交差性
    交差種: Mouse, Rat, Human, Pig
  • 免疫原

    Synthetic peptide:

    SVADTKGVKRRTL

    , corresponding to amino acids 499-511 of Mouse LDL Receptor

  • ポジティブ・コントロール
    • Rat placenta, mouse liver, RAW 264.7 cell lysate

アプリケーション

Our Abpromise guarantee covers the use of ab30532 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt Use at an assay dependent concentration. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
IHC-Fr Use at an assay dependent concentration. Used at a dilution of 1/200 for 1 hr on mouse aortic root (see Abreview for further details).
IHC-P 1/400.
WB 1/200. Predicted molecular weight: 95 kDa.

The protein is highly glycosylated through N- and O-linkages and thus migrates at 100 kDa to 160 kDa on SDS-PAGE.

ICC 1/100.

ターゲット情報

  • 機能Binds LDL, the major cholesterol-carrying lipoprotein of plasma, and transports it into cells by endocytosis. In order to be internalized, the receptor-ligand complexes must first cluster into clathrin-coated pits. In case of HIV-1 infection, functions as a receptor for extracellular Tat in neurons, mediating its internalization in uninfected cells.
  • 関連疾患Defects in LDLR are the cause of familial hypercholesterolemia (FH) [MIM:143890]; a common autosomal semi-dominant disease that affects about 1 in 500 individuals. The receptor defect impairs the catabolism of LDL, and the resultant elevation in plasma LDL-cholesterol promotes deposition of cholesterol in the skin (xanthelasma), tendons (xanthomas), and coronary arteries (atherosclerosis).
  • 配列類似性Belongs to the LDLR family.
    Contains 3 EGF-like domains.
    Contains 7 LDL-receptor class A domains.
    Contains 6 LDL-receptor class B repeats.
  • 翻訳後修飾N- and O-glycosylated.
    Ubiquitinated by MYLIP leading to degradation.
  • 細胞内局在Cell membrane. Endomembrane system. Membrane > clathrin-coated pit. Found distributed from the plasma membrane to intracellular compartments.
  • Information by UniProt
  • 参照データベース
  • 別名
    • FH antibody
    • FHC antibody
    • LDL R antibody
    • LDL receptor antibody
    • LDLCQ2 antibody
    • Ldlr antibody
    • LDLR_HUMAN antibody
    • Low Density Lipoprotein Receptor antibody
    • Low density lipoprotein receptor class A domain containing protein 3 antibody
    • Low density lipoprotein receptor familial hypercholesterolemia antibody
    • Low-density lipoprotein receptor antibody
    see all

Anti-LDL Receptor antibody 画像



  • Predicted band size : 95 kDa
    Lane 1: Rat placenta homogenate (60ug)
    Lane 2: Mouse liver homogenate (60ug)
    Lane 3: RAW 264.7 cell lysate (60ug)

    Lane 1: Rat placenta homogenate (60ug)
    Lane 2: Mouse liver homogenate (60ug)
    Lane 3: RAW 264.7 cell lysate (60ug)

  • ab30532 staining LDL receptor in human lung parenchyma.
    Left panel: with primary antibody at 1/400. Right panel: isotype control.
    Sections were stained using an automated system (DAKO Autostainer Plus), at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers citrate pH6.1. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

  • ICC/IF image of ab30532 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab30532, 1/400) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-LDL Receptor antibody (ab30532) 使用論文

This product has been referenced in:
  • Wagschal A  et al. Genome-wide identification of microRNAs regulating cholesterol and triglyceride homeostasis. Nat Med 21:1290-7 (2015). Read more (PubMed: 26501192) »
  • Cai K  et al. Silencing diacylglycerol kinase-theta expression reduces steroid hormone biosynthesis and cholesterol metabolism in human adrenocortical cells. Biochim Biophys Acta 1841:552-62 (2014). Human . Read more (PubMed: 24369117) »

See all 20 Publications for this product

Product Wall

Application Western blot
Sample Mouse Tissue lysate - whole (liver)
Gel Running Conditions Non-reduced Denaturing (7.5)
Loading amount 25 µg
Specification liver
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Nov 23 2016

Application Western blot
Sample Human Cell lysate - whole cell (SCC61 cells)
Gel Running Conditions Reduced Denaturing
Loading amount 48 µg
Specification SCC61 cells
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

投稿 Oct 21 2015

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (293T, HepG2, MEF)
Loading amount 20 µg
Specification 293T, HepG2, MEF
Gel Running Conditions Reduced Denaturing (8%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 7% · Temperature: 20°C
Username

Mr. Dongil Kim

Verified customer

投稿 Jan 16 2013


As we discussed, I will send you a positive control free of charge: ab7187 (RAW 264.7 whole cell lysate). This cell lysate gave a dark lower molecular weight band, the identity of which is unknown, along with bands at 110, 130, and 160 kDa corresp...

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Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this h...

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Thanks for your email.
I believe this replacement order has been shipped and delivered on Tuesday July 18. It was on Abcam order reference number 1128916 which contained a vial of ab61392.
I hope this is helpful. Please let me know if you hav...

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Thank you for contacting Abcam.

I have attached a western blot image showing that depending on the sample type that you are probing, you will get a number of different bands. Therefore, when running your western blot, you should expect to see ...

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I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for one vial of ab61392 and one vial of ab52818.

To check the status of th...

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Thank you for sending along the additional PO information. I have located that order for ab61392 on our system.
I am happy to send out the replacement antibodies. Since a couple options have been discussed for these replacement antibodies, I just ...

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Thanks for your reply.
Again I apologize for the trouble finding antibodies to 3-nitrotyrosine & the human LDL receptor. The antibodies you have ordered so far have very few complaints and are ordered relatively frequently so it is certainly an un...

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