製品の概要
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製品名Anti-LDL Receptor antibody
LDL Receptor 一次抗体 製品一覧 -
製品の詳細Chicken polyclonal to LDL Receptor
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由来種Chicken
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アプリケーション適用あり: WBmore details
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種交差性交差種: Recombinant fragment
交差が予測される動物種: Mouse, Rat, Human
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免疫原
Recombinant fragment, corresponding to amino acids 29-205 of Human LDL Receptor.
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ポジティブ･コントロール
- LDLR transfected 293 cell lysate.
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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バッファーPreservative: None
Constituents: PBS -
Concentration information loading... -
精製度Immunogen affinity purified
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ポリ/モノポリクローナル
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アイソタイプIgY
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研究分野
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipid metabolism
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Cholesterol Metabolism
アプリケーション
Our Abpromise guarantee covers the use of ab14056 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | 1/1000. Detects a band of approximately 68, 100 kDa. This antibody has been tested on recombinant protein only. We have not tested it on endogenous protein. |
ターゲット情報
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機能Binds LDL, the major cholesterol-carrying lipoprotein of plasma, and transports it into cells by endocytosis. In order to be internalized, the receptor-ligand complexes must first cluster into clathrin-coated pits. In case of HIV-1 infection, functions as a receptor for extracellular Tat in neurons, mediating its internalization in uninfected cells.
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関連疾患Defects in LDLR are the cause of familial hypercholesterolemia (FH) [MIM:143890]; a common autosomal semi-dominant disease that affects about 1 in 500 individuals. The receptor defect impairs the catabolism of LDL, and the resultant elevation in plasma LDL-cholesterol promotes deposition of cholesterol in the skin (xanthelasma), tendons (xanthomas), and coronary arteries (atherosclerosis).
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配列類似性Belongs to the LDLR family.
Contains 3 EGF-like domains.
Contains 7 LDL-receptor class A domains.
Contains 6 LDL-receptor class B repeats. -
翻訳後修飾N- and O-glycosylated.
Ubiquitinated by MYLIP leading to degradation. -
細胞内局在Cell membrane. Endomembrane system. Membrane > clathrin-coated pit. Found distributed from the plasma membrane to intracellular compartments.
- Information by UniProt
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参照データベース
- Entrez Gene: 3949 Human
- Entrez Gene: 16835 Mouse
- Entrez Gene: 300438 Rat
- Omim: 606945 Human
- SwissProt: P01130 Human
- SwissProt: P35951 Mouse
- SwissProt: P35952 Rat
- Unigene: 213289 Human
see all -
別名
- FH antibody
- FHC antibody
- LDL R antibody
see all
画像
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Western blot - Anti-LDL Receptor antibody (ab14056)Transiently transfected 293 cells probed with ab14056. Transiently transfected 293 cells probed with ab14056. -
Western blot - Anti-LDL Receptor antibody (ab14056)This image is courtesy of an anonymous AbreviewAnti-LDL Receptor antibody (ab14056) at 1/5000 dilution + CHO-cells transfected with human LDLR at 20 µg
Secondary
Goat Anti-Chicken IgY H&L (HRP) (ab6877) at 1/2000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Observed band size: 120,150 kDa (why is the actual band size different from the predicted?)
Exposure time: 2 minutes
プロトコール
参考文献
This product has been referenced in:
- Sato S et al. Anti-fibrotic efficacy of nintedanib in pulmonary fibrosis via the inhibition of fibrocyte activity. Respir Res 18:172 (2017). Read more (PubMed: 28915889) »
- Tong JG et al. Spatial and temporal epithelial ovarian cancer cell heterogeneity impacts Maraba virus oncolytic potential. BMC Cancer 17:594 (2017). Read more (PubMed: 28854921) »
レビューと Q&A
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"