Customer reviews and FAQs

製品の概要

  • 製品名
    Anti-LDL Receptor antibody
    LDL Receptor 一次抗体 製品一覧
  • 製品の詳細
    Chicken polyclonal to LDL Receptor
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Recombinant fragment
    交差が予測される動物種: Mouse, Rat, Human
  • 免疫原

    Recombinant fragment, corresponding to amino acids 29-205 of Human LDL Receptor.

  • ポジティブ･コントロール
    • LDLR transfected 293 cell lysate.

アプリケーション

Our Abpromise guarantee covers the use of ab14056 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000. Detects a band of approximately 68, 100 kDa.
This antibody has been tested on recombinant protein only. We have not tested it on endogenous protein.

ターゲット情報

  • 機能
    Binds LDL, the major cholesterol-carrying lipoprotein of plasma, and transports it into cells by endocytosis. In order to be internalized, the receptor-ligand complexes must first cluster into clathrin-coated pits. In case of HIV-1 infection, functions as a receptor for extracellular Tat in neurons, mediating its internalization in uninfected cells.
  • 関連疾患
    Defects in LDLR are the cause of familial hypercholesterolemia (FH) [MIM:143890]; a common autosomal semi-dominant disease that affects about 1 in 500 individuals. The receptor defect impairs the catabolism of LDL, and the resultant elevation in plasma LDL-cholesterol promotes deposition of cholesterol in the skin (xanthelasma), tendons (xanthomas), and coronary arteries (atherosclerosis).
  • 配列類似性
    Belongs to the LDLR family.
    Contains 3 EGF-like domains.
    Contains 7 LDL-receptor class A domains.
    Contains 6 LDL-receptor class B repeats.
  • 翻訳後修飾
    N- and O-glycosylated.
    Ubiquitinated by MYLIP leading to degradation.
  • 細胞内局在
    Cell membrane. Endomembrane system. Membrane > clathrin-coated pit. Found distributed from the plasma membrane to intracellular compartments.
  • Information by UniProt
  • 参照データベース
    see all
  • 別名
    • FH antibody
    • FHC antibody
    • LDL R antibody
    • LDL receptor antibody
    • LDLCQ2 antibody
    • Ldlr antibody
    • LDLR_HUMAN antibody
    • Low Density Lipoprotein Receptor antibody
    • Low density lipoprotein receptor class A domain containing protein 3 antibody
    • Low density lipoprotein receptor familial hypercholesterolemia antibody
    • Low-density lipoprotein receptor antibody
    see all

Anti-LDL Receptor antibody 画像

  • Western blot - LDL Receptor antibody (ab14056)
    Western blot - LDL Receptor antibody (ab14056)
    Transiently transfected 293 cells probed with ab14056.
  • Western blot - LDL Receptor antibody (ab14056)
    Western blot - LDL Receptor antibody (ab14056)
    Anti-LDL Receptor antibody (ab14056) at 1/5000 dilution + CHO-cells transfected with human LDLR at 20 µg

    Secondary
    Goat Anti-Chicken IgY H&L (HRP) (ab6877) at 1/2000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Observed band size : 120,150 kDa (why is the actual band size different from the predicted?)


    Exposure time : 2 minutes

    This image is courtesy of an anonymous Abreview

    See Abreview

プロトコール

Anti-LDL Receptor antibody (ab14056) 使用論文

This product has been referenced in:
  • Rojek A  et al. Liver-specific Aquaporin 11 knockout mice show rapid vacuolization of the rough endoplasmic reticulum in periportal hepatocytes after amino acid feeding. Am J Physiol Gastrointest Liver Physiol 304:G501-15 (2013). Mouse . Read more (PubMed: 23275615) »
  • Du F  et al. Novel Domain Interaction Regulates Secretion of Proprotein Convertase Subtilisin/Kexin Type 9 (PCSK9) Protein. J Biol Chem 286:43054-61 (2011). Read more (PubMed: 22027821) »

See all 17 Publications for this product

ab14056 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

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ab14056 の abreview を投稿する ab14056 に関する問い合わせをする

8 Abreviews or Q&A

10
Votes: Highest First

Western blot abreview for Anti-LDL Receptor antibody

 Good
Abreviews
Application
Western blot
Sample
Human Recombinant protein (CHO-cells transfected with human LDLR)
Loading amount
20 µg
Specification
CHO-cells transfected with human LDLR
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
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Abcam user community

Verified customer

投稿 Feb 22 2007

As we talked last week, I attached pictures of blots using both LDL receptor antibodies. The blots are quite confusing for us. Please let us know what you think.

Thank you for your enquiry. For ab14056, this was tested against a fusion protein corresponding to amino acids 29 to 205 of the human LDL receptor. Thus, the bands seen will be much smaller than for the full-length protein. Native LDLR will run as...

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i´m vervy interested in the Antibody of the LDL Receptor for FACS analyses. In the datasheets are only applications given for WB and ICC. I hope that you can give me information if i also can use the above listed products for FACS analyses. Thank you ...

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Thank you for your email. Neither of these antibodies have been tested yet for application in FACS. All tested applications are specified on Abcam product datasheets. If you decide to go ahead and purchase one of these products, please let us know how ...

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Hello I am a PHD student, working in the department of Clinical Microbilogy and Immunology, Charite, Berlin, Germany. Iam looking forword to order LDLr antibody, which I found in your website (catalog Number ab14056); but I would really want to ask wh...

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Thank you for your enquiry. I have been in touch with the source of this antibody and I have determined that neutralisation is not recommended as an application for this antibody. Although mouse and human LDL-r have good homology in sequence and th...

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BATCH NUMBER ? ORDER NUMBER 76364 DESCRIPTION OF THE PROBLEM No staining SAMPLE Rat Hepatocytes, Endothelial cells, 3T3 cells POSITIVE AND NEGATIVE CONTROLS USED Endothelial cells on Plastic (pos) Cells with IgY and secondar...

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Thank you for your enquiry and for taking your time to fill in the Questionnaire. We are very sorry to hear that you are having problem with this antibody. Could you please let us know the batch number you have received? It may well be that the ant...

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1. Please describe the problem (high background, wrong band size, more bands, no band etc)no signal not even a weak!! 2. On what material are you testing the antibody in WB? • Species? mouse • Cell extract/ Nuclear extract? cell lysate • Purifie...

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I'm sorry to hear ab14056 is giving your customer problems. I have had a good look at the protocol you sent and it looks fine, I would recommend sending him free of charge a new vial to try out. I hope this helps, please let me know if you need furthe...

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I bought the antibody two or three weeks ago. The antibody works very fine with rat cell extracts on western blot. I also tested the antibody on IHC, with acetone fixation and it also worked. Unfortunately, when I made aliquots of the antibody I spu...

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Thank you for your email, and I am very pleased to hear that ab14056 is working out for you in both Western blotting and IHC. I suggest that you submit a review (and get a gift certificate to Amazon.com in return!) - just go to the online datasheet for...

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Datasheet states that this antibody has been tested for application in IHC; can you be more sepcific regarding the conditions?

Thank you very much for your patience. I have just received the following information regarding this antibody: ab14056 was raised against a protein domain of 176 amino acids at the N-terminus and is expected to work in IHC. However, this has not yet be...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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