特異性Ab14519 shows strong reactivity to fully oxidized modifications of LDL including Cu2+ oxidized LDL, LDL(MDA), LDL(HOCL), but not to other oxidized proteins like HSA(MDA), HDL(MDA), HSA(HOCL), HDL(HOCL). The reaction to native LDL is weak, but detectable (approx. 20%). Minimally oxidized LDL gives a strong binding signal (> 80%).
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
追加情報ELISA: 1/500 - 1/5000.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
関連性When too much LDL cholesterol circulates in the blood, it can slowly build up in the inner walls of the arteries that feed the heart and brain. Together with other substances it can form plaque, a thick, hard deposit that can clog those arteries. This condition is known as atherosclerosis. Oxidized lipoproteins are formed by free radical damage to lipids that accumulate in macrophages and smooth muscle cells causing foam cell formation, an initial step in the disease.
Li R et al. Role of liver sinusoidal endothelial cells and stabilins in elimination of oxidized low-density lipoproteins. Am J Physiol Gastrointest Liver Physiol300:G71-81 (2011).
Read more (PubMed: 21030611) »
Dong Y et al. Activation of AMP-activated protein kinase inhibits oxidized LDL-triggered endoplasmic reticulum stress in vivo. Diabetes59:1386-96 (2010).
Read more (PubMed: 20299472) »