製品の概要

  • 製品名
  • 製品の詳細
    Goat polyclonal to LDB3
  • アプリケーション
    適用あり: IHC-P, WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Dog, Pig
  • 免疫原

    Synthetic peptide:

    KSQNKPEDEADE

    , corresponding to Internal sequence amino acids 251-262 of Human LDB3

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris saline, pH 7.3
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 特記事項(精製)
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab40840 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 3 - 6 µg/ml.
WB Use a concentration of 0.3 - 1 µg/ml. Predicted molecular weight: 77 kDa.

ターゲット情報

  • 機能
    May function as an adapter in striated muscle to couple protein kinase C-mediated signaling via its LIM domains to the cytoskeleton.
  • 組織特異性
    Expressed primarily in skeletal muscle and to a lesser extent in heart. Also detected in brain and placenta.
  • 関連疾患
    Defects in LDB3 are the cause of cardiomyopathy dilated type 1C (CMD1C) [MIM:601493]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
    Defects in LDB3 are the cause of left ventricular non-compaction type 3 (LVNC3) [MIM:601493]. Left ventricular non-compaction is characterized by numerous prominent trabeculations and deep intertrabecular recesses in hypertrophied and hypokinetic segments of the left ventricle.
    Defects in LDB3 are the cause of myopathy myofibrillar ZASP-related (MFM-ZASP) [MIM:609452]. A neuromuscular disorder characterized by distal and proximal muscle weakness with signs of cardiomyopathy and neuropathy.
  • 配列類似性
    Contains 3 LIM zinc-binding domains.
    Contains 1 PDZ (DHR) domain.
  • 細胞内局在
    Cytoplasm > perinuclear region. Cell projection > pseudopodium. Cytoplasm > cytoskeleton. Cytoplasm > myofibril > sarcomere > Z line. Localized to the cytoplasm around nuclei and pseudopodia of undifferentiated cells and detected throughout the myotubes of differentiated cells. Colocalizes with ACTN2 at the Z-lines.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CMD1C antibody
    • CYPHER antibody
    • HGNC:15710 antibody
    • KIAA01613 antibody
    • KIAA0613 antibody
    • Ldb3 antibody
    • LDB3_HUMAN antibody
    • LDB3Z1 antibody
    • LDB3Z4 antibody
    • LIM domain binding 3 antibody
    • LIM domain binding protein 3 antibody
    • LIM domain-binding protein 3 antibody
    • LVNC3 antibody
    • ORACLE antibody
    • PDLIM6 antibody
    • PDZ and LIM domain 6 antibody
    • Protein cypher antibody
    • Z band alternatively spliced PDZ motif antibody
    • Z band alternatively spliced PDZ motif protein antibody
    • Z-band alternatively spliced PDZ-motif protein antibody
    • ZASP antibody
    see all

画像

  • Anti-LDB3 antibody (ab40840) at 0.01 µg/ml + Human Muscle lysate (35µg protein in RIPA buffer).

    Predicted band size : 77 kDa
  • ab40840 at 3.8 µg/ml staining LBD3 in Human Skeletal muscle by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Steamed antigen retrieval with citrate pH 6, AP-staining.

参考文献

This product has been referenced in:
  • Ulbricht A  et al. Induction and adaptation of chaperone-assisted selective autophagy CASA in response to resistance exercise in human skeletal muscle. Autophagy 11:538-46 (2015). WB . Read more (PubMed: 25714469) »
  • Johnson EK  et al. Identification of new dystroglycan complexes in skeletal muscle. PLoS One 8:e73224 (2013). Mouse . Read more (PubMed: 23951345) »

See all 3 Publications for this product

レビューと Q&A

Thank you again for your call yesterday and for letting us know about the trouble with the new lot of this antibody.
I've heard back from the lab, and the new batch of ab40840 passed QC testing, however it did need to be used at a much higher conc...

Read More

Thank you for your call today and for letting us know about the trouble with this antibody.
As we discussed, I'm sending a free of charge replacement vial on the order ***, which should arrive tomorrow. I've also asked the lab if they are producin...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

登録