Binds to the LIM domain of a wide variety of LIM domain-containing transcription factors. May regulate the transcriptional activity of LIM-containing proteins by determining specific partner interactions. May play a role in the development of motor neurons. Acts synergistically with LHX1/LIM1 in axis formation and activation of gene expression. Acts with LMO2 in the regulation of red blood cell development, maintaining erythroid precursors in an immature state.
Expressed in a wide range of adult tissues including brain, heart, skeletal muscle, colon, thymus, spleen, kidney, liver, small intestine, lung and peripheral blood leukocytes.
Belongs to the LDB family.
The dimerization domain is located in the N-terminus.
Ubiquitinated by RLIM/RNF12, leading to its degradation by the proteasome.
Immunofluorescence analysis of paraformaldheyde-fixed HeLa cells using ab96799 at a dilution of 1/200.
Western blot - Anti-LDB1 antibody (ab96799)
All lanes : Anti-LDB1 antibody (ab96799) at 1/1000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell lysate Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 47 kDa
Western blot - LDB1 antibody (ab96799)
Anti-LDB1 antibody (ab96799) at 1/1000 dilution + Raji whole cell lysate at 30 µg
LDB1 was immunoprecipitated from 1000 µg 293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate with ab96799. Western blot was performed from the immunoprecipitate using 2.5 μg of ab96799 at 1/1000 dilution.
Lane A: 20 μg 293T whole cell lysate/extract. Lane B: Control with 2.5 μg of preimmune rabbit IgG. Lane C: ab96799 IP in 293T (12% SDS-PAGE).
ab96799 staining LDB1 (red) in mouse brain tissue section immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde and blocked with 5% serum for 1 hour at 25°C. Samples were incubated with primary antibody (1/300 dilution in PBST) for 12 hours at 4°C. An Alexa Fluor® 594-conjugated donkey anti-rabbit IgG polyclonal (1/1000 dilution) was used as the secondary antibody.
Stanulovic VS et al. LMO2 is required for TAL1 DNA binding activity and initiation of definitive haematopoiesis at the haemangioblast stage. Nucleic Acids Res45:9874-9888 (2017).
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