製品の概要

  • 製品名Anti-Lck antibody [LCK-01]
    Lck 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [LCK-01] to Lck
  • 特異性The antibody recognizes defined epitope (aa 22-36) on p56lck.
  • アプリケーション適用あり: ICC, Flow Cyt, IP, WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide, corresponding to amino acids 22-36 of Human Lck p56.

  • ポジティブ・コントロール
    • Jurkat cell lysate.
  • 特記事項


    Lck (p56lck), a member of the Src family of non-receptor tyrosine protein kinases, is expressed predominantly in T cells. Lck function is critical both for T cell development in the thymus and activation of mature T cells in the periphery by antigen. The activity of Lck is known to be regulated by phosphorylation of two conserved tyrosine residues, Tyr-505 (equivalent to Tyr-529 in c-Src) and Tyr-394 (equivalent to Tyr-418 in c-Src).

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーPreservative: 15mM Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • 精製度>95% by SDS-PAGE
  • 特記事項(精製)Purified from ascites using protein A-affinity chromatography.
  • 一次抗体 備考Lck (p56lck), a member of the Src family of non-receptor tyrosine protein kinases, is expressed predominantly in T cells. Lck function is critical both for T cell development in the thymus and activation of mature T cells in the periphery by antigen. The activity of Lck is known to be regulated by phosphorylation of two conserved tyrosine residues, Tyr-505 (equivalent to Tyr-529 in c-Src) and Tyr-394 (equivalent to Tyr-418 in c-Src).
  • ポリ/モノモノクローナル
  • クローン名LCK-01
  • アイソタイプIgG1
  • 研究分野

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab8075 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration.

ターゲット情報

  • 機能Tyrosine kinase that plays an essential role for the selection and maturation of developing T-cell in the thymus and in mature T-cell function. Is constitutively associated with the cytoplasmic portions of the CD4 and CD8 surface receptors and plays a key role in T-cell antigen receptor(TCR)-linked signal transduction pathways. Association of the TCR with a peptide antigen-bound MHC complex facilitates the interaction of CD4 and CD8 with MHC class II and class I molecules, respectively, and thereby recruits the associated LCK to the vicinity of the TCR/CD3 complex. LCK then phosphorylates tyrosines residues within the immunoreceptor tyrosines-based activation motifs (ITAMs) in the cytoplasmic tails of the TCRgamma chains and CD3 subunits, initiating the TCR/CD3 signaling pathway. In addition, contributes to signaling by other receptor molecules. Associates directly with the cytoplasmic tail of CD2, and upon engagement of the CD2 molecule, LCK undergoes hyperphosphorylation and activation. Also plays a role in the IL2 receptor-linked signaling pathway that controls T-cell proliferative response. Binding of IL2 to its receptor results in increased activity of LCK. Is expressed at all stages of thymocyte development and is required for the regulation of maturation events that are governed by both pre-TCR and mature alpha beta TCR. Phosphorylates RUNX3.
  • 組織特異性Expressed specifically in lymphoid cells.
  • 関連疾患Note=A chromosomal aberration involving LCK is found in leukemias. Translocation t(1;7)(p34;q34) with TCRB.
  • 配列類似性Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.
    Contains 1 protein kinase domain.
    Contains 1 SH2 domain.
    Contains 1 SH3 domain.
  • ドメインThe SH2 domain mediates interaction with SQSTM1. Interaction is regulated by Ser-59 phosphorylation.
  • 翻訳後修飾Phosphorylated on Tyr-394, which increases enzymatic activity (By similarity). Phosphorylated on Tyr-505, which decreases activity.
  • 細胞内局在Cytoplasm. Cell membrane. Present in lipid rafts in an unactive form.
  • Information by UniProt
  • 参照データベース
  • 製品の状態This protein is known to be similar in amino acid sequence to HCK (P08631), FYN (P06241), YES1 (P07947), SRC (P12931), and LYN (P07948). Therefore, cross-reactivity with these homologous proteins may be observed. We would be happy to provide immunogen alignment information upon request.
  • 別名
    • IMD22 antibody
    • LCK antibody
    • Lck p56 antibody
    • LCK proto-oncogene, Src family tyrosine kinase antibody
    • LCK_HUMAN antibody
    • Leukocyte C-terminal Src kinase antibody
    • LSK antibody
    • Lymphocyte cell specific protein tyrosine kinase antibody
    • Lymphocyte cell-specific protein-tyrosine kinase antibody
    • Lymphocyte specific protein tyrosine kinase antibody
    • Membrane associated protein tyrosine kinase antibody
    • Oncogene lck antibody
    • P56 LCK antibody
    • p56(LSTRA) protein tyrosine kinase antibody
    • p56-LCK antibody
    • p56lck antibody
    • pp58 lck antibody
    • pp58lck antibody
    • Protein YT16 antibody
    • Proto oncogene tyrosine protein kinase LCK antibody
    • Proto-oncogene Lck antibody
    • Protooncogene tyrosine protein kinase LCK antibody
    • T cell specific protein tyrosine kinase antibody
    • T cell-specific protein-tyrosine kinase antibody
    • T lymphocyte specific protein tyrosine kinase p56lck antibody
    • Tyrosine-protein kinase Lck antibody
    • YT 16 antibody
    • YT16 antibody
    see all

Anti-Lck antibody [LCK-01] (ab8075) 使用論文

This product has been referenced in:
  • Romagnoli P  et al. A potential role for protein tyrosine kinase p56(lck) in rheumatoid arthritis synovial fluid T lymphocyte hyporesponsiveness. Int Immunol 13:305-12 (2001). Read more (PubMed: 11222499) »
  • Cebecauer M  et al. Incorporation of leucocyte GPI-anchored proteins and protein tyrosine kinases into lipid-rich membrane domains of COS-7 cells. Biochem Biophys Res Commun 243:706-10 (1998). Read more (PubMed: 9500981) »

See all 2 Publications for this product

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