製品の概要

  • 製品名Anti-LCAT antibody [EPR1384Y]
    LCAT 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR1384Y] to LCAT
  • アプリケーション適用あり: Flow Cyt, IP, WB, IHC-P, ICC/IFmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide corresponding to residues on the C terminus of human LCAT.

  • ポジティブ・コントロール
    • WB: Human serum IHC-P: Human brain tissue ICC/IF: HeLa cells
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Alternative versions available:

    Anti-LCAT antibody (HRP) [EPR1384Y] (ab201345)

アプリケーション

Our Abpromise guarantee covers the use of ab51060 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt 1/10. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
IP 1/100.
WB 1/10000. Detects a band of approximately 62 kDa (predicted molecular weight: 50 kDa).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/250.

ターゲット情報

  • 機能Central enzyme in the extracellular metabolism of plasma lipoproteins. Synthesized mainly in the liver and secreted into plasma where it converts cholesterol and phosphatidylcholines (lecithins) to cholesteryl esters and lysophosphatidylcholines on the surface of high and low density lipoproteins (HDLs and LDLs). The cholesterol ester is then transported back to the liver. Has a preference for plasma 16:0-18:2 or 18:O-18:2 phosphatidylcholines. Also produced in the brain by primary astrocytes, and esterifies free cholesterol on nascent APOE-containing lipoproteins secreted from glia and influences cerebral spinal fluid (CSF) APOE- and APOA1 levels. Together with APOE and the cholesterol transporter ABCA1, plays a key role in the maturation of glial-derived, nascent lipoproteins. Required for remodeling high-density lipoprotein particles into their spherical forms.
  • 組織特異性Expressed mainly in brain, liver and testes. Secreted into plasma and cerebral spinal fluid. In liver, expressed in HEPG2 hepatocytes.
  • 関連疾患Defects in LCAT are the cause of lecithin-cholesterol acyltransferase deficiency (LCATD) [MIM:245900]; also called Norum disease. LCATD is a disorder of lipoprotein metabolism characterized by inadequate esterification of plasmatic cholesterol. Two clinical forms are recognized: familial LCAT deficiency and fish-eye disease. Familial LCAT deficiency is associated with a complete absence of alpha and beta LCAT activities and results in esterification anomalies involving both HDL (alpha-LCAT activity) and LDL (beta-LCAT activity). It causes a typical triad of diffuse corneal opacities, target cell hemolytic anemia, and proteinuria with renal failure.
    Defects in LCAT are a cause of fish-eye disease (FED) [MIM:136120]; also known as dyslipoproteinemic corneal dystrophy or alpha-LCAT deficiency. FED is due to a partial LCAT deficiency that affects only alpha-LCAT activity. It is characterized by low plasma HDL and corneal opacities due to accumulation of cholesterol deposits in the cornea ('fish-eye').
  • 配列類似性Belongs to the AB hydrolase superfamily. Lipase family.
  • 翻訳後修飾O- and N-glycosylated. O-glycosylation on Thr-431 and Ser-433 consists of sialylated galactose beta 1-->3N-acetylgalactosamine structures. N-glycosylated sites contain sialylated triantennary and/or biantennary complex structures.
  • 細胞内局在Secreted. Secreted into blood plasma. Produced in astrocytes and secreted into cerebral spinal fluid.
  • Information by UniProt
  • 参照データベース
  • 別名
    • LCAT antibody
    • LCAT_HUMAN antibody
    • Lecithin cholesterol acyltransferase antibody
    • Lecithin-cholesterol acyltransferase antibody
    • Phosphatidylcholine sterol acyltransferase antibody
    • Phosphatidylcholine-sterol acyltransferase antibody
    • Phospholipid cholesterol acyltransferase antibody
    • Phospholipid-cholesterol acyltransferase antibody
    see all

Anti-LCAT antibody [EPR1384Y] 画像

  • Anti-LCAT antibody [EPR1384Y] (ab51060) at 1/10000 dilution + Human serum at 10 µg

    Secondary
    Goat anti-Rabbit HRP at 1/2000 dilution

    Predicted band size : 50 kDa
    Observed band size : 62 kDa (why is the actual band size different from the predicted?)
  • Ab51060 (1/100) staining LCAT in paraffin embedded human brain tissue by Immunohistochemistry.
  • Immunofluorescent staining of HeLa cells using ab51060 (1/100).

Anti-LCAT antibody [EPR1384Y] (ab51060) 使用論文

This product has been referenced in:
  • Bogan RL & Hennebold JD The reverse cholesterol transport system as a potential mediator of luteolysis in the primate corpus luteum. Reproduction 139:163-76 (2010). Read more (PubMed: 19776099) »

See 1 Publication for this product

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