Anti-LATS2 抗体 (ab111054)
Key features and details
- Rabbit polyclonal to LATS2
- Suitable for: IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-LATS2 antibody
LATS2 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to LATS2 -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, ICC/IFmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse -
免疫原
Synthetic peptide derived from an internal sequence of Human LATS2.
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ポジティブ・コントロール
- HepG2 cells; Human skeletal muscle tissue.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49.1% PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride
without Mg2+ and Ca2+ -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
ab111054 was affinity-purified from Rabbit antiserum by affinity-chromatography using epitope-specific immunogen. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab111054の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF |
1/100 - 1/500.
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特記事項 |
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IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
1/100 - 1/500. |
ターゲット情報
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機能
Negative regulator of YAP1 in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. Acts as a tumor suppressor which plays a critical role in centrosome duplication, maintenance of mitotic fidelity and genomic stability. Negatively regulates G1/S transition by down-regulating cyclin E/CDK2 kinase activity. Negative regulator of the androgen receptor. -
組織特異性
Expressed at high levels in heart and skeletal muscle and at lower levels in all other tissues examined. -
配列類似性
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 protein kinase domain.
Contains 1 UBA domain. -
翻訳後修飾
Autophosphorylated and phosphorylated during M-phase and the G1/S-phase of the cell cycle. Phosphorylated and activated by STK3. -
細胞内局在
Cytoplasm > cytoskeleton > centrosome. Cytoplasm. Cytoplasm > cytoskeleton > spindle pole. Co-localizes with STK6 at the centrosomes during interphase, early prophase and cytokinesis. Migrates to the spindle poles during mitosis, and to the midbody during cytokinesis. - Information by UniProt
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参照データベース
- Entrez Gene: 26524 Human
- Entrez Gene: 50523 Mouse
- Omim: 604861 Human
- SwissProt: Q9NRM7 Human
- SwissProt: Q7TSJ6 Mouse
- Unigene: 78960 Human
- Unigene: 347899 Mouse
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別名
- FLJ13161 antibody
- Kinase phosphorylated during mitosis protein antibody
- KPM antibody
see all
画像
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ab111054 at 1/100 dilution staining LATS2 in HepG2 cells by Immunofluorescence. The image on the right is treated with the synthesized peptide.
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ab110541 at 1/50 dilution staining LATS2 in paraffin-embedded Human Skeletal muscle tissue by Immunohistochemistry. The image on the right is treated with the synthesized peptide.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (5)
ab111054 は 5 報の論文で使用されています。
- Wang L et al. Toxoplasma gondii causes changes in the host's expression of cancer-associated miRNAs. Oncol Lett 23:149 (2022). PubMed: 35350589
- Sun X & Qiu X LncRNA TPTEP1 inhibited the proliferation and metastasis of non-small cell lung cancer cells by targeting miR-761/LATS2 axis. Am J Transl Res 13:8653-8669 (2021). PubMed: 34539985
- Zhao C et al. Large tumor suppressor 2 is a prognostic biomarker and correlated with immune infiltrates in colorectal cancer. Bioengineered 12:11648-11661 (2021). PubMed: 34699318
- Liu ZJ et al. MiR-15b-5b Regulates the Proliferation of Prostate Cancer PC-3 Cells via Targeting LATS2. Cancer Manag Res 12:10669-10678 (2020). PubMed: 33149674
- Su X et al. ELK1-induced upregulation of long non-coding RNA MIR100HG predicts poor prognosis and promotes the progression of osteosarcoma by epigenetically silencing LATS1 and LATS2. Biomed Pharmacother 109:788-797 (2019). PubMed: 30551532