Anti-Late Inflammatory Macrophages 抗体 [25 F 9] (FITC) (ab15689)

製品の概要

  • 製品名
    Anti-Late Inflammatory Macrophages antibody [25 F 9] (FITC)
  • 製品の詳細
    Mouse monoclonal [25 F 9] to Late Inflammatory Macrophages (FITC)
  • 標識
    FITC. Ex: 493nm, Em: 528nm
  • アプリケーション
    適用あり: IHC-Pmore details
  • 種交差性
    交差種: Human, Pig, Rhesus monkey
  • 免疫原

    Unfortunately, this information is considered to be commercially sensitive

  • ポジティブ・コントロール
    • Tested with subpopulation of macrophages of rhesus monkey, pig alveolar macrophages and Kupffer cells.
  • 特記事項


    Monoclonal antibody 25F9 is associated with fully differentiated tissue macrophages both in normal and diseased tissues, independently of the presence or absence of inflammation. The antibody is also suitable for staining macrophages from bronchial lavage fluids and similar techniques. It is very useful for macrophage phenotyping, particularly for the classification of late inflammatory stages. It is used in tissue sections and in smears, for the characterization of tumorous tissues and the monitoring of macrophage cell cultures.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー
    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: PBS, 1% BSA
  • Concentration information loading...
  • 精製度
    IgG fraction
  • 一次抗体 備考
    Monoclonal antibody 25F9 is associated with fully differentiated tissue macrophages both in normal and diseased tissues, independently of the presence or absence of inflammation. The antibody is also suitable for staining macrophages from bronchial lavage fluids and similar techniques. It is very useful for macrophage phenotyping, particularly for the classification of late inflammatory stages. It is used in tissue sections and in smears, for the characterization of tumorous tissues and the monitoring of macrophage cell cultures.
  • ポリ/モノ
    モノクローナル
  • クローン名
    25 F 9
  • アイソタイプ
    IgG1
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab15689 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/200.

ターゲット情報

  • 細胞内局在
    .
  • 別名
    • Late stage inflammatory macrophages antibody

画像

  • ab15689 at 1/150 staining sections of monkey spleen by IHC-P

    The tissue section was incubated with ab15689 for 1 hr at 23°C followed by a secondary incubation with an HRP-conjugated Goat anti-Mouse antibody.  

    The blocking agent was 5% serum, incubated for 20 min at 23°C.

    See Abreview

プロトコール

参考文献

ab15689 has not yet been referenced specifically in any publications.

レビューと Q&A

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Monkey Tissue sections (Spleen)
Specification
Spleen
Fixative
Formaldehyde
Antigen retrieval step
None
Permeabilization
No
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 23°C
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投稿 Nov 12 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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