製品の概要

  • 製品名Anti-LAT (phospho Y191) antibody
    LAT 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to LAT (phospho Y191)
  • 特異性Detects endogenous levels of LAT only when phosphorylated at tyrosine 191. This antibody detects LAT phosphorylated on Tyrosine 191 in isoform 2 (SwissProt: O43561-2), and Tyrosine 220 in isoform 1 (SwissProt: O43561).
  • アプリケーション適用あり: WB, IHC-P, ELISAmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat
  • 免疫原

    Synthetic phosphopeptide from around the phosphorylation site of tyrosine 191 (REYPVN) of human LAT

  • ポジティブ・コントロール
    • Human colon carcinoma tissue NIH3T3 cell extract

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • バッファーPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 特記事項(精製)Affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab59197 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Detects a band of approximately 28 kDa (predicted molecular weight: 28 kDa).
IHC-P Use at an assay dependent concentration.
ELISA 1/5000.

ターゲット情報

  • 機能Required for TCR (T-cell antigen receptor)- and pre-TCR-mediated signaling, both in mature T-cells and during their development. Involved in FCGR3 (low affinity immunoglobulin gamma Fc region receptor III)-mediated signaling in natural killer cells and FCER1 (high affinity immunoglobulin epsilon receptor)-mediated signaling in mast cells. Couples activation of these receptors and their associated kinases with distal intracellular events such as mobilization of intracellular calcium stores, PKC activation, MAPK activation or cytoskeletal reorganization through the recruitment of PLCG1, GRB2, GRAP2, and other signaling molecules.
  • 組織特異性Expressed in thymus, T-cells, NK cells, mast cells and, at lower levels, in spleen. Present in T-cells but not B-cells (at protein level).
  • 翻訳後修飾Phosphorylated on tyrosines by ZAP-70 upon TCR activation, or by SYK upon other immunoreceptor activation; which leads to the recruitment of multiple signaling molecules. Is one of the most prominently tyrosine-phosphorylated proteins detected following TCR engagement. May be dephosphorylated by PTPRJ.
    Palmitoylation of Cys-26 and Cys-29 is required for raft targeting and efficient phosphorylation.
  • 細胞内局在Cell membrane. Present in lipid rafts.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 36 kDa phospho tyrosine adapter protein antibody
    • 36 kDa phospho-tyrosine adapter protein antibody
    • 36 kDa phospho-tyrosine adaptor protein antibody
    • LAT 1 antibody
    • lat antibody
    • LAT_HUMAN antibody
    • LAT1 antibody
    • Linker for activation of T cells antibody
    • Linker for activation of T cells family member 1 antibody
    • linker for activation of T cells, transmembrane adaptor antibody
    • Linker for activation of T-cells family member 1 antibody
    • p36 38 antibody
    • p36-38 antibody
    • pp36 antibody
    see all

Anti-LAT (phospho Y191) antibody 画像

  • Immunohistochemical analysis of paraffin embedded human colon carcinoma tissue using ab59197 at 1/50 dilution. Left: untreated. Right: sample treated with immunising peptide.
  • All lanes : Anti-LAT (phospho Y191) antibody (ab59197) at 1/500 dilution

    Lane 1 : NIH3T3 cells treated with PMA
    Lane 2 : NIH3T3 cells treated with PMA with phosphopeptide


    Predicted band size : 28 kDa
    Observed band size : 28 kDa

Anti-LAT (phospho Y191) antibody (ab59197) 使用論文

This product has been referenced in:
  • Crites TJ  et al. TCR Microclusters pre-exist and contain molecules necessary for TCR signal transduction. J Immunol 193:56-67 (2014). WB ; Mouse . Read more (PubMed: 24860189) »
  • Kota DJ  et al. TSG-6 produced by hMSCs delays the onset of autoimmune diabetes by suppressing Th1 development and enhancing tolerogenicity. Diabetes 62:2048-58 (2013). WB . Read more (PubMed: 23349496) »

See all 2 Publications for this product

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