Anti-LAT (phospho Y191) 抗体 (ab59197)
Key features and details
- Rabbit polyclonal to LAT (phospho Y191)
- Suitable for: WB, IHC-P, ELISA
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-LAT (phospho Y191) antibody
LAT 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to LAT (phospho Y191) -
由来種
Rabbit -
特異性
Detects endogenous levels of LAT only when phosphorylated at tyrosine 191. This antibody detects LAT phosphorylated on Tyrosine 191 in isoform 2 (SwissProt: O43561-2), and Tyrosine 220 in isoform 1 (SwissProt: O43561). -
アプリケーション
適用あり: WB, IHC-P, ELISAmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat -
免疫原
Synthetic peptide corresponding to Human LAT aa 150-250 (phospho Y191).
Database link: O43561 -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg+2 and Ca+2 -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
Affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab59197の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/500 - 1/1000. Detects a band of approximately 28 kDa (predicted molecular weight: 28 kDa).
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IHC-P |
Use at an assay dependent concentration.
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ELISA |
Use at an assay dependent concentration.
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特記事項 |
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WB
1/500 - 1/1000. Detects a band of approximately 28 kDa (predicted molecular weight: 28 kDa). |
IHC-P
Use at an assay dependent concentration. |
ELISA
Use at an assay dependent concentration. |
ターゲット情報
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機能
Required for TCR (T-cell antigen receptor)- and pre-TCR-mediated signaling, both in mature T-cells and during their development. Involved in FCGR3 (low affinity immunoglobulin gamma Fc region receptor III)-mediated signaling in natural killer cells and FCER1 (high affinity immunoglobulin epsilon receptor)-mediated signaling in mast cells. Couples activation of these receptors and their associated kinases with distal intracellular events such as mobilization of intracellular calcium stores, PKC activation, MAPK activation or cytoskeletal reorganization through the recruitment of PLCG1, GRB2, GRAP2, and other signaling molecules. -
組織特異性
Expressed in thymus, T-cells, NK cells, mast cells and, at lower levels, in spleen. Present in T-cells but not B-cells (at protein level). -
翻訳後修飾
Phosphorylated on tyrosines by ZAP-70 upon TCR activation, or by SYK upon other immunoreceptor activation; which leads to the recruitment of multiple signaling molecules. Is one of the most prominently tyrosine-phosphorylated proteins detected following TCR engagement. May be dephosphorylated by PTPRJ.
Palmitoylation of Cys-26 and Cys-29 is required for raft targeting and efficient phosphorylation. -
細胞内局在
Cell membrane. Present in lipid rafts. - Information by UniProt
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参照データベース
- Entrez Gene: 27040 Human
- Entrez Gene: 16797 Mouse
- Entrez Gene: 81511 Rat
- Omim: 602354 Human
- SwissProt: O43561 Human
- SwissProt: O54957 Mouse
- SwissProt: O70601 Rat
- Unigene: 632179 Human
see all -
別名
- 36 kDa phospho tyrosine adapter protein antibody
- 36 kDa phospho-tyrosine adapter protein antibody
- 36 kDa phospho-tyrosine adaptor protein antibody
see all
画像
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Immunohistochemical analysis of paraffin embedded human colon carcinoma tissue using ab59197 at 1/50 dilution. Left: untreated. Right: sample treated with immunising peptide.
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All lanes : Anti-LAT (phospho Y191) antibody (ab59197) at 1/500 dilution
Lane 1 : NIH3T3 cells treated with PMA
Lane 2 : NIH3T3 cells treated with PMA with phosphopeptide
Predicted band size: 28 kDa
Observed band size: 28 kDa
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab59197 は 3 報の論文で使用されています。
- Bustos-Morán E et al. Microtubule-associated protein-4 controls nanovesicle dynamics and T cell activation. J Cell Sci 130:1217-1223 (2017). PubMed: 28209780
- Crites TJ et al. TCR Microclusters pre-exist and contain molecules necessary for TCR signal transduction. J Immunol 193:56-67 (2014). WB ; Mouse . PubMed: 24860189
- Kota DJ et al. TSG-6 produced by hMSCs delays the onset of autoimmune diabetes by suppressing Th1 development and enhancing tolerogenicity. Diabetes 62:2048-58 (2013). WB . PubMed: 23349496