製品の概要

  • 製品名Anti-LAT (phospho Y171) antibody
    LAT 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to LAT (phospho Y171)
  • 特異性ab73205 detects endogenous levels of LAT only when phosphorylated at tyrosine 171.
  • アプリケーション適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse
  • 免疫原

    Synthetic phosphopeptide derived from human LAT around the phosphorylation site of tyrosine 171 (DDYPVN)

  • ポジティブ・コントロール
    • Extracts from Jurkat cells treated with UV (5mins).

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • バッファーPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 特記事項(精製)ab73205 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab73205 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Detects a band of approximately 40 kDa (predicted molecular weight: 28 kDa).
ELISA 1/10000.

ターゲット情報

  • 機能Required for TCR (T-cell antigen receptor)- and pre-TCR-mediated signaling, both in mature T-cells and during their development. Involved in FCGR3 (low affinity immunoglobulin gamma Fc region receptor III)-mediated signaling in natural killer cells and FCER1 (high affinity immunoglobulin epsilon receptor)-mediated signaling in mast cells. Couples activation of these receptors and their associated kinases with distal intracellular events such as mobilization of intracellular calcium stores, PKC activation, MAPK activation or cytoskeletal reorganization through the recruitment of PLCG1, GRB2, GRAP2, and other signaling molecules.
  • 組織特異性Expressed in thymus, T-cells, NK cells, mast cells and, at lower levels, in spleen. Present in T-cells but not B-cells (at protein level).
  • 翻訳後修飾Phosphorylated on tyrosines by ZAP-70 upon TCR activation, or by SYK upon other immunoreceptor activation; which leads to the recruitment of multiple signaling molecules. Is one of the most prominently tyrosine-phosphorylated proteins detected following TCR engagement. May be dephosphorylated by PTPRJ.
    Palmitoylation of Cys-26 and Cys-29 is required for raft targeting and efficient phosphorylation.
  • 細胞内局在Cell membrane. Present in lipid rafts.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 36 kDa phospho tyrosine adapter protein antibody
    • 36 kDa phospho-tyrosine adapter protein antibody
    • 36 kDa phospho-tyrosine adaptor protein antibody
    • LAT 1 antibody
    • lat antibody
    • LAT_HUMAN antibody
    • LAT1 antibody
    • Linker for activation of T cells antibody
    • Linker for activation of T cells family member 1 antibody
    • linker for activation of T cells, transmembrane adaptor antibody
    • Linker for activation of T-cells family member 1 antibody
    • p36 38 antibody
    • p36-38 antibody
    • pp36 antibody
    see all

Anti-LAT (phospho Y171) antibody 画像

  • All lanes : Anti-LAT (phospho Y171) antibody (ab73205) at 1/500 dilution

    Lane 1 : Extracts from Jurkat cells treated with UV (5 min)
    Lane 2 : Extracts from Jurkat cells treated with UV (5 min) with immunising phosphopeptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 28 kDa
    Observed band size : 40 kDa (why is the actual band size different from the predicted?)

Anti-LAT (phospho Y171) antibody (ab73205) 使用論文

This product has been referenced in:
  • Bronk CC  et al. NF-?B is crucial in proximal T-cell signaling for calcium influx and NFAT activation. Eur J Immunol N/A:N/A (2014). Read more (PubMed: 25251667) »
  • Driessens G  et al. Beta-catenin inhibits T cell activation by selective interference with linker for activation of T cells-phospholipase C-gamma1 phosphorylation. J Immunol 186:784-90 (2011). Read more (PubMed: 21149602) »

See all 2 Publications for this product

Product Wall

There are currently no Abreviews or Questions for ab73205.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"