製品の概要

  • 製品名Anti-LAS1L antibody
    LAS1L 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to LAS1L
  • アプリケーション適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Horse
  • 免疫原

    A region within synthetic peptide: PASSSFGSEA KAQQQEEQGS VNDVKEEEKE EKEVLPDQVE EEEENDDQEE, corresponding to C terminal amino acids 541-590 of Human LAS1L

  • ポジティブ・コントロール
    • HepG2 cell lysate

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab49279 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 83 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1:312500.

ターゲット情報

  • 機能Involved in the biogenesis of the 60S ribosomal subunit. Required for maturation of the 28S rRNA. Functions as a component of the Five Friends of Methylated CHTOP (5FMC) complex; the 5FMC complex is recruited to ZNF148 by methylated CHTOP, leading to desumoylation of ZNF148 and subsequent transactivation of ZNF148 target genes.
  • 配列類似性Belongs to the LAS1 family.
  • 細胞内局在Nucleus > nucleolus. Nucleus > nucleoplasm. Cytoplasm. Mainly found in the nucleoplasm, with low levels detected in the cytoplasmic and chromatin fractions (By similarity). Localizes mainly to the granular component, the region implicated in the later steps of rRNA processing and subunit assembly and export.
  • Information by UniProt
  • 参照データベース
  • 別名
    • dJ475B7.2 antibody
    • FLJ12525 antibody
    • FLJ30133 antibody
    • FLJ45274 antibody
    • LAS1 like (S. cerevisiae) antibody
    • Las1 like antibody
    • LAS1L antibody
    • LAS1L_HUMAN antibody
    • Protein LAS1 homolog antibody
    • Ribosomal biogenesis protein LAS1L antibody
    • RP3 475B7.2 antibody
    see all

Anti-LAS1L antibody 画像

  • Anti-LAS1L antibody (ab49279) at 1.25 µg/ml + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit Ig at 1/50000 dilution
    Developed using the ECL technique

    Predicted band size : 83 kDa
    Observed band size : 70 kDa (why is the actual band size different from the predicted?)

Anti-LAS1L antibody (ab49279) 使用論文

ab49279 has not yet been referenced specifically in any publications.

Product Wall

Abreviews
Application Western blot
Sample Human Cell lysate - whole cell (LUNG CANCER)
Loading amount 200000 cells
Specification LUNG CANCER
Gel Running Conditions Reduced Denaturing (10 %)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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投稿 Jun 29 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"