製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to LARP1
  • アプリケーション
    適用あり: WB, IP, IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Chimpanzee, Rhesus monkey, Gorilla
  • 免疫原

    Synthetic peptide corresponding to a region between residues 275 and 325 of Human LARP1 (NP_291029.2)

  • ポジティブ・コントロール
    • HeLa whole cell lysate.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    Preservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab86359 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2000 - 1/10000. Predicted molecular weight: 124 kDa.
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

ターゲット情報

  • 関連性
    LARP1 belongs to the LARP family and contains 1 HTH La-type RNA-binding domain.
  • 参照データベース
  • 別名
    • 1810024J12Rik antibody
    • 3110040D16Rik antibody
    • KIAA0731 antibody
    • La related protein antibody
    • La ribonucleoprotein domain family, member 1 antibody
    • LARP antibody
    • MGC19556 antibody
    • mKIAA0731 antibody
    • rCG_32791 antibody
    • RGD1306683 antibody
    • RP23-293I3.7 antibody
    see all

画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon carcinoma tissue labelling LARP1 with ab86359 at 1/200 (1µg/ml). Detection: DAB.
  • All lanes : Anti-LARP1 antibody (ab86359) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg


    Predicted band size : 124 kDa


    Exposure time : 10 seconds

参考文献

This product has been referenced in:
  • Müller AC  et al. Identifying Kinase Substrates via a Heavy ATP Kinase Assay and Quantitative Mass Spectrometry. Sci Rep 6:28107 (2016). Read more (PubMed: 27346722) »
  • Fonseca BD  et al. La-related Protein 1 (LARP1) Represses Terminal Oligopyrimidine (TOP) mRNA Translation Downstream of mTOR Complex 1 (mTORC1). J Biol Chem 290:15996-6020 (2015). WB . Read more (PubMed: 25940091) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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