Anti-LARG 抗体 (ab86095)
Key features and details
- Rabbit polyclonal to LARG
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-LARG antibody
LARG 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to LARG -
由来種
Rabbit -
アプリケーション
適用あり: WB, IPmore details -
種交差性
交差種: Human
交差が予測される動物種: Chimpanzee, Rhesus monkey, Gorilla, Orangutan -
免疫原
Synthetic peptide, corresponding to a region between residues 1350 and 1400 of human LARG (NP_056128.1)
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ポジティブ・コントロール
- HeLa whole cell lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
ab86095 was affinity purified using an epitope specific to LARG immobilized on solid support. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab86095の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/2000 - 1/10000. Detects a band of approximately 173 kDa (predicted molecular weight: 173 kDa).
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IP |
Use at 2-5 µg/mg of lysate.
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特記事項 |
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WB
1/2000 - 1/10000. Detects a band of approximately 173 kDa (predicted molecular weight: 173 kDa). |
IP
Use at 2-5 µg/mg of lysate. |
ターゲット情報
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機能
May play a role in the regulation of RhoA GTPase by guanine nucleotide-binding alpha-12 (GNA12) and alpha-13 (GNA13). Acts as guanine nucleotide exchange factor (GEF) for RhoA GTPase and may act as GTPase-activating protein (GAP) for GNA12 and GNA13. -
組織特異性
Ubiquitously expressed. Isoform 2 is found in jejunum and testis. -
関連疾患
A chromosomal aberration involving ARHGEF12 may be a cause of acute leukemia. Translocation t(11;11)(q23;23) with KMT2A/MLL1. -
配列類似性
Contains 1 DH (DBL-homology) domain.
Contains 1 PDZ (DHR) domain.
Contains 1 PH domain.
Contains 1 RGSL (RGS-like) domain. -
細胞内局在
Cytoplasm. Membrane. Translocated to the membrane upon stimulation. - Information by UniProt
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参照データベース
- Entrez Gene: 23365 Human
- Omim: 604763 Human
- SwissProt: Q9NZN5 Human
- Unigene: 24598 Human
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別名
- ARHGC_HUMAN antibody
- ARHGEF12 antibody
- KIAA0382 antibody
see all
画像
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All lanes : Anti-LARG antibody (ab86095) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Predicted band size: 173 kDa
Observed band size: 173 kDa
Exposure time: 3 minutes -
Detection of LARG by Western Blot of Immunprecipitate.
ab86095 at 1µg/ml staining LARG in HeLa whole cell lysate immunoprecipitated using ab86095 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 30 seconds.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab86095 は 1 報の論文で使用されています。
- Osborne LD et al. TGF-ß regulates LARG and GEF-H1 during EMT to affect stiffening response to force and cell invasion. Mol Biol Cell 25:3528-40 (2014). WB . PubMed: 25143398