製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to LANPL
  • 特異性
    This antibody does not cross-react with PHAPI or PHAP12a.
  • アプリケーション
    適用あり: ICC/IF, WB, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide based on sequence near C-terminus (Human).

  • ポジティブ・コントロール
    • A549 or HepG2 whole cell lysate.
  • 特記事項
    Stable for one year.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab5993 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 1 µg/ml.
WB Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 35 kDa.Can be blocked with LANPL peptide (ab6242).
IHC-P Use a concentration of 2 µg/ml.

ターゲット情報

  • 機能
    Inhibits activity of protein phosphatase 2A (PP2A). Does not inhibit protein phosphatase 1. May play a role in cerebellar development and synaptogenesis process by modulating PP2A activity.
  • 組織特異性
    Expressed in peripheral blood leukocytes, colon, small intestine, prostate, thymus, spleen, skeletal muscle, liver and kidney.
  • 配列類似性
    Belongs to the ANP32 family.
    Contains 4 LRR (leucine-rich) repeats.
    Contains 1 LRRCT domain.
  • 翻訳後修飾
    Phosphorylated. The phosphorylation is nuclear localization signal (NLS)-dependent.
  • 細胞内局在
    Cytoplasm. Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Acidic (leucine rich) nuclear phosphoprotein 32 family member E antibody
    • Acidic leucine-rich nuclear phosphoprotein 32 family member E antibody
    • AN32E_HUMAN antibody
    • anp32e antibody
    • Cerebellar postnatal development protein 1 antibody
    • LANP like protein antibody
    • LANP-L antibody
    • LANP-like protein antibody
    • LANPL antibody
    • Leucine rich acidic nuclear protein like antibody
    • MGC5350 antibody
    see all

画像

  • Western blot analysis of LANPL expression in human A549 (A) and HepG2 (B) cells, and rat testis (C) with ab5993 at 1 µg/ml.
  • ab5993 at 2µg/ml staining LANPL in human small intestine tissue by IHC

  • ICC/IF image of ab5993 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab5993, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Immunofluorescence of PHAP III in Human Small Intestine cells using ab5993 at 20 ug/ml.

参考文献

This product has been referenced in:
  • Mao Z  et al. Anp32e, a higher eukaryotic histone chaperone directs preferential recognition for H2A.Z. Cell Res 24:389-99 (2014). Human . Read more (PubMed: 24613878) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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