Anti-LANCL2 抗体 (ab88860)
Key features and details
- Mouse polyclonal to LANCL2
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-LANCL2 antibody
LANCL2 一次抗体 製品一覧 -
製品の詳細
Mouse polyclonal to LANCL2 -
由来種
Mouse -
アプリケーション
適用あり: WB, ICC/IFmore details -
種交差性
交差種: Human -
免疫原
Recombinant full length protein within Human LANCL2. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
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ポジティブ・コントロール
- WB: A431 cell lysate; LANCL2 transfected 293T cell lysate ICC/IF: HeLa cell
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.40
Constituent: 100% PBS -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab88860の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use a concentration of 1 µg/ml. Predicted molecular weight: 51 kDa.
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ICC/IF |
Use a concentration of 10 µg/ml.
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特記事項 |
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WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 51 kDa. |
ICC/IF
Use a concentration of 10 µg/ml. |
ターゲット情報
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機能
Necessary for abscisic acid (ABA) binding on the cell membrane and activation of the ABA signaling pathway in granulocytes. -
組織特異性
Expressed in brain and testis. -
配列類似性
Belongs to the LanC-like protein family. -
翻訳後修飾
Myristoylated. Essential for membrane association. -
細胞内局在
Nucleus. Cytoplasm. Cell membrane. Localizes to the juxta-nuclear vesicles. Associates with the cortical actin cytoskeleton. Cholesterol depletion by methyl-beta-cyclodextrin causes partial dissociation from the cell membrane in vitro and an enhanced cell detachment from the matrix in vivo. Membrane-association is important for the increased cellular sensitivity to an anticancer drug. - Information by UniProt
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参照データベース
- Entrez Gene: 55915 Human
- SwissProt: Q9NS86 Human
- Unigene: 595384 Human
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別名
- G protein coupled receptor 69B antibody
- GPR69B antibody
- LanC (bacterial lantibiotic synthetase component C) like 2 antibody
see all
画像
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Anti-LANCL2 antibody (ab88860) at 1 µg/ml + A431 cell lysate at 50 µg
Predicted band size: 51 kDa
Observed band size: ~48 kDa why is the actual band size different from the predicted? -
All lanes : Anti-LANCL2 antibody (ab88860) at 1 µg/ml
Lane 1 : LANCL2 transfected 293T cell lysate
Lane 2 : non transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Predicted band size: 51 kDa
Observed band size: ~45 kDa why is the actual band size different from the predicted? -
ab88860, at 10 µg/ml, staining LANCL2 in paraformaldehyde fixed, permeabilized HeLa cell by Immunofluorescence.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (1)
ab88860 は 1 報の論文で使用されています。
- Lou Y et al. Akt kinase LANCL2 functions as a key driver in EGFR-mutant lung adenocarcinoma tumorigenesis. Cell Death Dis 12:170 (2021). PubMed: 33568630