Anti-LAMP2B 抗体 - Lysosome Marker (ab18529)

製品の概要

  • 製品名Anti-LAMP2B antibody - Lysosome Marker
    LAMP2B 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to LAMP2B - Lysosome Marker
  • 特異性This product has low sequence homology with Mouse LAMP2B (67%), and high sequence homology with Mouse LAMP2C (91.7%).
  • アプリケーション適用あり: ICC/IF, IHC-P, WBmore details
  • 種交差性
    交差種: Cow, Human
  • 免疫原

    Synthetic peptide corresponding to Human LAMP2B aa 350 to the C-terminus (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). LAMP2 has 3 distinct isoforms, LAMP2A, 2B & 2C which are expressed in different tissues and differ in sequence at the extreme C-terminus.
    Database link: P13473
    (Peptide available as ab23321)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in the following whole cell lysates: HeLa, HEK293. This antibody gave a positive signal in the following Methanol fixed cell lines: HeLa. It also gave a positive signal in Human skin tissue sections.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーPreservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab18529 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 1 µg/ml.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 110 kDa (predicted molecular weight: 110 kDa).

Abcam recommends using milk as the blocking agent.

ターゲット情報

  • 機能Implicated in tumor cell metastasis. May function in protection of the lysosomal membrane from autodigestion, maintenance of the acidic environment of the lysosome, adhesion when expressed on the cell surface (plasma membrane), and inter- and intracellular signal transduction. Protects cells from the toxic effects of methylating mutagens.
  • 組織特異性Isoform LAMP-2A is highly expressed in placenta, lung and liver, less in kidney and pancreas, low in brain and skeletal muscle. Isoform LAMP-2B is highly expressed in skeletal muscle, less in brain, placenta, lung, kidney and pancreas, very low in liver.
  • 関連疾患Danon disease
  • 配列類似性Belongs to the LAMP family.
  • 翻訳後修飾O- and N-glycosylated; some of the 16 N-linked glycans are polylactosaminoglycans.
  • 細胞内局在Cell membrane. Endosome membrane. Lysosome membrane. This protein shuttles between lysosomes, endosomes, and the plasma membrane.
  • Information by UniProt
  • 参照データベース
  • 製品の状態Alternative splicing produces 3 isoforms.
  • 別名
    • CD_antigen=CD107b antibody
    • CD107 antigen like family member B antibody
    • CD107 antigen-like family member B antibody
    • CD107b antibody
    • LAMP 2 antibody
    • Lamp 2b antibody
    • LAMP-2 antibody
    • LAMP2 antibody
    • LAMP2_HUMAN antibody
    • LAMPB antibody
    • LGP110 antibody
    • Lysosomal associated membrane protein 2 antibody
    • Lysosome associated membrane glycoprotein 2 antibody
    • Lysosome-associated membrane glycoprotein 2 antibody
    • Lysosome-associated membrane protein 2 antibody
    • MAC3 antibody
    see all

Anti-LAMP2B antibody - Lysosome Marker 画像

  • All lanes : Anti-LAMP2B antibody - Lysosome Marker (ab18529) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : HEK293 (Human) Whole Cell Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed at 1/50000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 110 kDa
    Observed band size : 110 kDa
    Additional bands at : 42 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 4 minutes

    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% Milk before being incubated with ab18529 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.

    LAMP2B undergoes extensive glycosylation so ab18529 detects a band of 110 kDa by Western blot.

  • ICC/IF image of ab18529 stained human HeLa cells. The cells were methanol fixed (5 min) and incubated with the antibody (ab18529, 1µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Image-iTTM FX Signal Enhancer was used as the primary blocking agent, 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 WGA was used to label plasma membranes (red).

  • IHC image of ab18529 staining in human skin formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab18529, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-LAMP2B antibody - Lysosome Marker (ab18529) 使用論文

This product has been referenced in:

See all 5 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing (10% gel)
Sample Human Cell lysate - whole cell (BA9 - Cortical area)
Specification BA9 - Cortical area
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Dr. Martin Broadstock

Verified customer

投稿 Aug 16 2013

Thank you very much for your interest in our products.

We do not offer free or trial sizes of our products simply because we carry over 100,000 items and having multiple sizes would be logistically impossible with our automated freezers. Read More

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Cow Cell (endothelial cells)
Specification endothelial cells
Fixative acetone/methanol
Permeabilization No
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
Username

Abcam user community

Verified customer

投稿 Sep 17 2008

Application Western blot
Sample Mouse Cell lysate - whole cell (embryonic fibriblast)
Loading amount 50 µg
Specification embryonic fibriblast
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 22°C
Username

Dr. Eeva-Liisa Eskelinen

Verified customer

投稿 Dec 11 2007

Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (cultured hippocampal astrocytes)
Specification cultured hippocampal astrocytes
Fixative Paraformaldehyde
Permeabilization Yes - 0.25% Triton X-100 for 5 min
Blocking step BSA as blocking agent for 20 minute(s) · Concentration: 1% · Temperature: 20°C
Username

Abcam user community

Verified customer

投稿 Aug 16 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"