製品の概要

  • 製品名Anti-LAMP2 antibody
    LAMP2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to LAMP2
  • アプリケーション適用あり: WB, IHC-Pmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant protein fragment contain a sequence corresponding to a region within amino acids 70-237 of Human LAMP2 (NP_054701).

  • ポジティブ・コントロール
    • A549, HeLa, HepG2, HCT116 whole cell lysate; BT474 xenograft

法規制情報

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab101325 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/3000. Predicted molecular weight: 45 kDa.
IHC-P 1/100 - 1/500. Suggested antigen retrieval using heat mediated 10mM Citrate buffer or Tris-EDTA buffer (pH 8.0)

ターゲット情報

  • 機能Implicated in tumor cell metastasis. May function in protection of the lysosomal membrane from autodigestion, maintenance of the acidic environment of the lysosome, adhesion when expressed on the cell surface (plasma membrane), and inter-and intracellular signal transduction. Protects cells from the toxic effects of methylating mutagens.
  • 組織特異性Isoform LAMP-2A is highly expressed in placenta, lung and liver, less in kidney and pancreas, low in brain and skeletal muscle. Isoform LAMP-2B is highly expressed in skeletal muscle, less in brain, placenta, lung, kidney and pancreas, very low in liver.
  • 関連疾患Defects in LAMP2 are the cause of Danon disease (DAND) [MIM:300257]; also known as glycogen storage disease type 2B (GSD2B). DAND is a lysosomal glycogen storage disease characterized by the clinical triad of cardiomyopathy, vacuolar myopathy and mental retardation. It is often associated with an accumulation of glycogen in muscle and lysosomes.
  • 配列類似性Belongs to the LAMP family.
  • 翻訳後修飾O- and N-glycosylated; some of the 16 N-linked glycans are polylactosaminoglycans.
  • 細胞内局在Cell membrane. Endosome membrane. Lysosome membrane. This protein shuttles between lysosomes, endosomes, and the plasma membrane.
  • Information by UniProt
  • 参照データベース
  • 製品の状態Alternative splicing produces 3 isoforms.
  • 別名
    • CD107 antigen-like family member B antibody
    • CD107b antibody
    • LAMP 2 antibody
    • LAMP 2C antibody
    • LAMP-2 antibody
    • LAMP2 antibody
    • LAMP2_HUMAN antibody
    • LAMPB antibody
    • LGP110 antibody
    • Lysosomal associated membrane protein 2 antibody
    • Lysosome associated membrane protein 2 antibody
    • Lysosome-associated membrane glycoprotein 2 antibody
    • Lysosome-associated membrane protein 2 antibody
    • MAC3 antibody
    see all

Anti-LAMP2 antibody 画像

  • All lanes : Anti-LAMP2 antibody (ab101325) at 1/500 dilution (LAMP2 antibody)

    Lane 1 : A431
    Lane 2 : HeLa
    Lane 3 : HepG2

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 45 kDa
    Observed band size : 105 kDa (why is the actual band size different from the predicted?)

    Various whole cell extracts were separated by 7.5% SDS-PAGE.

  • Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using LAMP2(ab101325) antibody (Green) at 1/200 dilution.

  • Anti-LAMP2 antibody (ab101325) at 1/3000 dilution + HCT116 whole cell lysate at 30 µg

    Predicted band size : 45 kDa
    10% SDS PAGE
  • Immunohistochemical analysis of paraffin-embedded BT474 xenograft, using ab101325 at 1/500 dilution.

Anti-LAMP2 antibody (ab101325) 使用論文

ab101325 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"