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Rat liver lysosomal membrane preparations
Our Abpromise guarantee covers the use of ab13523 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 120 kDa.|
|IP||Use at an assay dependent dilution.|
|ICC||1/100 - 1/750.|
|ICC/IF||Use at an assay dependent concentration. PubMed: 21536677|
ab13523 at 1/100 dilution staining LAMP1 in LAMP1 transfected HeLa cells by Immunocytochemistry/ immunofluorescence. A Alexa Fluor® 568 conjugated goat anti mouse was used at 1/50 dilution as secondary.
Image from Banno A et al, J Biol Chem. 2012 Apr 20;287(17):13799-812. Epub 2012 Feb 18, Fig 4. DOI 10.1074/jbc.M112.341214 April 20, 2012 The Journal of Biological Chemistry, 287, 13799-13812.ab13523 used in Western Blot. Lysates prepared from CHO cells stably expressing aIIbß3 integrins (A5 cells) were used.