製品の概要

  • 製品名
    Anti-LAMP1 antibody [LY1C6]
    LAMP1 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [LY1C6] to LAMP1
  • 由来種
    Mouse
  • アプリケーション
    適用あり: WB, IP, ICC, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Rat, Hamster
  • 免疫原

    Rat liver lysosomal membrane preparations

  • ポジティブ・コントロール
    • Highly purified rat lysosomal membrane preparations.
  • 特記事項
    For maximum product recovery centrifuge the product vial before removing cap.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab13523 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 120 kDa.
IP Use at an assay dependent dilution.
ICC 1/100 - 1/750.
ICC/IF Use at an assay dependent concentration. PubMed: 21536677

ターゲット情報

  • 機能
    Presents carbohydrate ligands to selectins. Also implicated in tumor cell metastasis.
  • 配列類似性
    Belongs to the LAMP family.
  • 翻訳後修飾
    O- and N-glycosylated; some of the 18 N-linked glycans are polylactosaminoglycans.
  • 細胞内局在
    Cell membrane. Endosome membrane. Lysosome membrane. This protein shuttles between lysosomes, endosomes, and the plasma membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CD107 antigen like family member A antibody
    • CD107 antigen-like family member A antibody
    • CD107a antibody
    • CD107a antigen antibody
    • LAMP 1 antibody
    • LAMP-1 antibody
    • LAMP1 antibody
    • LAMP1_HUMAN antibody
    • LAMPA antibody
    • LGP120 antibody
    • lgpA antibody
    • Lysosomal membrane glycoprotein 120KD antibody
    • Lysosomal Associated Membrane Protein 1 antibody
    • Lysosome associated membrane glycoprotein 1 antibody
    • Lysosome-associated membrane glycoprotein 1 antibody
    • Lysosome-associated membrane protein 1 antibody
    • OTTHUMP00000040663 antibody
    see all

画像

  • ab13523 used in Western Blot. Lysates prepared from CHO cells stably expressing aIIbß3 integrins (A5 cells) were used.
    WCL, N/IC, C, P, and PM indicate whole cell lysates, nuclear/intact cell, cytosolic, high-speed pellet, and plasma membrane fractions, respectively.
  • ab13523 at 1/100 dilution staining LAMP1 in LAMP1 transfected HeLa cells by Immunocytochemistry/ immunofluorescence. A Alexa Fluor® 568 conjugated goat anti mouse was used at 1/50 dilution as secondary.

参考文献

This product has been referenced in:
  • Wang B  et al. Essential control of mitochondrial morphology and function by chaperone-mediated autophagy through degradation of PARK7. Autophagy 12:1215-28 (2016). Read more (PubMed: 27171370) »
  • Sun H  et al. Resistance of glioma cells to nutrient-deprived microenvironment can be enhanced by CD133-mediated autophagy. Oncotarget 7:76238-76249 (2016). WB ; Rat . Read more (PubMed: 27780926) »

See all 17 Publications for this product

レビューと Q&A

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 20 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 22°C
Sample
Monkey Cell (cos7)
Specification
cos7
Permeabilization
Yes - 2% TritonX100
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

投稿 Aug 13 2014

Product Anti-LAMP1 antibody [LY1C6] (ab13523) is an unconjugated Ab, and does not contain any conjugated fluorophores or dyes. For successful ICC/IF staining of tissues using ab13523, a secondary Ab must be used that contains a conjugated fluorophore ...

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Application
Immunocytochemistry/ Immunofluorescence
Sample
Hamster Cell (BHK 21)
Specification
BHK 21
Fixative
Paraformaldehyde
Blocking step
BSA as blocking agent for 20 minute(s) · Concentration: 0.2%
Username

Dr. Giuseppe Balistreri

Verified customer

投稿 Apr 05 2007

For ICC/IF applications with product Anti-LAMP1 antibody [LY1C6] (ab13523), we recommend our standard ICC/IF protocol.

Protocol details and additional ICC/IF information are linked below:
...

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Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry
Sample
Dog Cell lysate - whole cell (MDCK cell line)
Specification
MDCK cell line
Fixative
Formaldehyde
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2%
Username

Mrs. Kun Liu

Verified customer

投稿 Sep 02 2005

Unfortunately, we have not yet tested reactivity of Anti-LAMP1 antibody [LY1C6] (ab13523) against human samples. As such, use of this Ab with human samples is not yet covered by our ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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