製品の概要

  • 製品名Anti-LAMP1 antibody [H4A3] (FITC)
    LAMP1 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [H4A3] to LAMP1 (FITC)
  • 標識FITC. Ex: 493nm, Em: 528nm
  • アプリケーション適用あり: Flow Cyt, IHC-Frmore details
  • 種交差性
    交差種: Human
  • 免疫原

    The details of the immunogen for this antibody are not available.

  • ポジティブ・コントロール
    • IHC-Fr: human colon sections

アプリケーション

Our Abpromise guarantee covers the use of ab25406 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt Use 5µl for 106 cells. ab91356-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IHC-Fr Use at an assay dependent concentration.

ターゲット情報

  • 機能Presents carbohydrate ligands to selectins. Also implicated in tumor cell metastasis.
  • 配列類似性Belongs to the LAMP family.
  • 翻訳後修飾O- and N-glycosylated; some of the 18 N-linked glycans are polylactosaminoglycans.
  • 細胞内局在Cell membrane. Endosome membrane. Lysosome membrane. This protein shuttles between lysosomes, endosomes, and the plasma membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CD107 antigen like family member A antibody
    • CD107 antigen-like family member A antibody
    • CD107a antibody
    • CD107a antigen antibody
    • LAMP 1 antibody
    • LAMP-1 antibody
    • LAMP1 antibody
    • LAMP1_HUMAN antibody
    • LAMPA antibody
    • LGP120 antibody
    • lgpA antibody
    • Lysosomal membrane glycoprotein 120KD antibody
    • Lysosomal Associated Membrane Protein 1 antibody
    • Lysosome associated membrane glycoprotein 1 antibody
    • Lysosome-associated membrane glycoprotein 1 antibody
    • Lysosome-associated membrane protein 1 antibody
    • OTTHUMP00000040663 antibody
    see all

Anti-LAMP1 antibody [H4A3] (FITC) 画像

  • Overlay histogram showing HT1080 cells stained with ab25406 (red line). The cells were fixed with 4% paraformaldehydel (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab25406, 0.5µg/1x106 cells) for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 (1µg/1x106 cells ). Acquisition of >5,000 events was performed. This antibody gave a positive signal in HT1080 cells fixed with 80% methano/permeabilized in 0.1% PBS-Tween used under the same conditions.

Anti-LAMP1 antibody [H4A3] (FITC) (ab25406) 使用論文

This product has been referenced in:
  • Patel PD  et al. Paralog-selective Hsp90 inhibitors define tumor-specific regulation of HER2. Nat Chem Biol 9:677-84 (2013). ICC/IF . Read more (PubMed: 23995768) »

See 1 Publication for this product

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