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RabMAb

Anti-LAMP1 抗体 [EPR4204] (ab108597)

製品の概要

  • 製品名
    Anti-LAMP1 antibody [EPR4204]
    LAMP1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR4204] to LAMP1
  • アプリケーション
    適用あり: WB, IHC-Pmore details
    適用なし: Flow Cyt,ICC or IP
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide corresponding to residues in Human LAMP1.

  • ポジティブ・コントロール
    • HeLa, JAR, Jurkat, and A431 cell lysates; Human kidney tissue
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

アプリケーション

Our Abpromise guarantee covers the use of ab108597 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/10000. Detects a band of approximately 120 kDa (predicted molecular weight: 45 kDa).
IHC-P 1/100 - 1/250.
  • 追加情報
    Is unsuitable for Flow Cyt,ICC or IP.
  • ターゲット情報

    • 機能
      Presents carbohydrate ligands to selectins. Also implicated in tumor cell metastasis.
    • 配列類似性
      Belongs to the LAMP family.
    • 翻訳後修飾
      O- and N-glycosylated; some of the 18 N-linked glycans are polylactosaminoglycans.
    • 細胞内局在
      Cell membrane. Endosome membrane. Lysosome membrane. This protein shuttles between lysosomes, endosomes, and the plasma membrane.
    • Information by UniProt
    • 参照データベース
    • 別名
      • CD107 antigen like family member A antibody
      • CD107 antigen-like family member A antibody
      • CD107a antibody
      • CD107a antigen antibody
      • LAMP 1 antibody
      • LAMP-1 antibody
      • LAMP1 antibody
      • LAMP1_HUMAN antibody
      • LAMPA antibody
      • LGP120 antibody
      • lgpA antibody
      • Lysosomal membrane glycoprotein 120KD antibody
      • Lysosomal Associated Membrane Protein 1 antibody
      • Lysosome associated membrane glycoprotein 1 antibody
      • Lysosome-associated membrane glycoprotein 1 antibody
      • Lysosome-associated membrane protein 1 antibody
      • OTTHUMP00000040663 antibody
      see all

    画像

    • Immunohistochemical analysis of LAMP1 in paraffin embedded Human kidney tissue, using ab108597 at a dilution of 1/100.
    • All lanes : Anti-LAMP1 antibody [EPR4204] (ab108597) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : JAR cell lysate
      Lane 3 : Jurkat cell lysate
      Lane 4 : A431 cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 45 kDa

    参考文献

    This product has been referenced in:
    • Wu X  et al. Mitochondrial proteomic analysis of human host cells infected with H3N2 swine influenza virus. J Proteomics 91:136-50 (2013). WB ; Human . Read more (PubMed: 23856606) »

    See 1 Publication for this product

    レビューと Q&A

    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Loading amount
    20 µg
    Gel Running Conditions
    Reduced Denaturing (10% gel)
    Sample
    Human Cell lysate - whole cell (Cortical tissue)
    Specification
    Cortical tissue
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Username

    Dr. Martin Broadstock

    Verified customer

    投稿 Jun 03 2013

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (BA9 cortical sections)
    Specification
    BA9 cortical sections
    Fixative
    Paraformaldehyde
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citric acid
    Permeabilization
    Yes - 0.05% Tween-20
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
    Username

    Dr. Martin Broadstock

    Verified customer

    投稿 May 17 2013

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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