Rabbit monoclonal [EPR4204] to LAMP1
Synthetic peptide corresponding to residues in Human LAMP1.
HeLa, JAR, Jurkat, and A431 cell lysates; Human kidney tissue
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab ® patents
This product is a recombinant rabbit monoclonal antibody.
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Preservative: 0.01% Sodium azide Constituents: 50% Glycerol, 0.05% BSA
Tissue culture supernatant
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000 - 1/10000. Detects a band of approximately 120 kDa (predicted molecular weight: 45 kDa).
1/100 - 1/250.
Is unsuitable for Flow Cyt,ICC or IP.
Presents carbohydrate ligands to selectins. Also implicated in tumor cell metastasis.
Belongs to the LAMP family.
O- and N-glycosylated; some of the 18 N-linked glycans are polylactosaminoglycans.
Cell membrane. Endosome membrane. Lysosome membrane. This protein shuttles between lysosomes, endosomes, and the plasma membrane.
Information by UniProt
CD107 antigen like family member A antibody
CD107 antigen-like family member A antibody
Western blot - Anti-LAMP1 antibody [EPR4204] (ab108597)
All lanes :
Anti-LAMP1 antibody [EPR4204] (ab108597) at 1/1000 dilution
Lane 1 :
Wild-type HAP1 whole cell lysate
Lane 2 :
LAMP1 knockout HAP1 whole cell lysate
Lane 3 :
MCF7 whole cell lysate
Lane 4 :
Human Kidney whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size:
Observed band size:
110 kDa (
why is the actual band size different from the predicted?
Lanes 1 - 4: Merged signal (red and green). Green - ab108597 observed at 110 kDa. Red - loading control, ab18058, observed at 130 kDa.
ab108597 was shown to recognize LAMP1 in wild-type HAP1 cells as signal was lost at the expected MW in LAMP1 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and LAMP1 knockout samples were subjected to SDS-PAGE. ab108597 and
ab18058 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye ® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye ® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - LAMP1 antibody [EPR4204] (ab108597)
Immunohistochemical analysis of LAMP1 in paraffin embedded Human kidney tissue, using ab108597 at a dilution of 1/100.
Western blot - LAMP1 antibody [EPR4204] (ab108597)
All lanes : Anti-LAMP1 antibody [EPR4204] (ab108597) at 1/1000 dilution Lane 1 : HeLa cell lysate Lane 2 : JAR cell lysate Lane 3 : Jurkat cell lysate Lane 4 : A431 cell lysate Lysates/proteins at 10 µg per lane. Predicted band size: 45 kDa
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