Anti-LAMP1 抗体 - Lysosome Marker (ab30687)
Key features and details
- Rabbit polyclonal to LAMP1 - Lysosome Marker
- Suitable for: ICC
- Reacts with: Drosophila melanogaster
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-LAMP1 antibody - Lysosome Marker
LAMP1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to LAMP1 - Lysosome Marker -
由来種
Rabbit -
アプリケーション
適用あり: ICCmore details
適用なし: WB -
種交差性
交差種: Drosophila melanogaster -
免疫原
Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Fruit fly (Drosophila melanogaster) LAMP1.
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ポジティブ・コントロール
- ICC: Drosophila S2 Schneider cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab30687の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC |
Use a concentration of 0.2 µg/ml.
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特記事項 |
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ICC
Use a concentration of 0.2 µg/ml. |
ターゲット情報
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機能
Presents carbohydrate ligands to selectins. Also implicated in tumor cell metastasis. -
配列類似性
Belongs to the LAMP family. -
翻訳後修飾
O- and N-glycosylated; some of the 18 N-linked glycans are polylactosaminoglycans. -
細胞内局在
Cell membrane. Endosome membrane. Lysosome membrane. This protein shuttles between lysosomes, endosomes, and the plasma membrane. - Information by UniProt
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別名
- CD107 antigen like family member A antibody
- CD107 antigen-like family member A antibody
- CD107a antibody
see all
画像
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ab30687 staining LAMP1 in Drosophila S2 Schneider cells. The cells were fixed with 100% methanol (5 min) then permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab30687 at 0.2μg/ml concentration and ab44928 (Mouse monoclonal to Tubulin) at 0.2μg/ml concentration overnight at 4°C followed by a further incubation at room temperature for 1h with a goat secondary antibody to rabbit IgG (Alexa Fluor® 488) (ab150081) at 2μg/ml (shown in green) and a goat secondary antibody to mouse IgG (Alexa Fluor® 594) (ab150120) at 2μg/ml (shown in red). Nuclear DNA was labelled in blue with DAPI.
Image was taken with a confocal microscope (Leica-Microsystems TCS SP8).
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (23)
ab30687 は 23 報の論文で使用されています。
- Tao X et al. Phenylbutyrate modulates polyamine acetylase and ameliorates Snyder-Robinson syndrome in a Drosophila model and patient cells. JCI Insight 7:N/A (2022). PubMed: 35801587
- Linnemannstöns K et al. Microscopic and biochemical monitoring of endosomal trafficking and extracellular vesicle secretion in an endogenous in vivo model. J Extracell Vesicles 11:e12263 (2022). PubMed: 36103151
- Sidisky JM et al. Mayday sustains trans-synaptic BMP signaling required for synaptic maintenance with age. Elife 10:N/A (2021). PubMed: 33667157
- Witte L et al. The kinesin motor Klp98A mediates apical to basal Wg transport. Development 147:N/A (2020). PubMed: 32665246
- Chittoor-Vinod VG et al. Dietary Amino Acids Impact LRRK2-Induced Neurodegeneration in Parkinson's Disease Models. J Neurosci 40:6234-6249 (2020). PubMed: 32605938