製品の概要

  • 製品名
    Anti-LAMP1 antibody (Cy3 ®)
    LAMP1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to LAMP1 (Cy3 ®)
  • 標識
    Cy3 ®. Ex: 552nm, Em: 565nm
  • アプリケーション
    適用あり: ICC/IFmore details
  • 種交差性
    交差種: Mouse, Rat, Hamster, Human
    交差が予測される動物種: Chicken, Cow, Pig, Xenopus laevis, Zebrafish, Woodchuck
  • 免疫原

    Synthetic peptide:

    GRKRSHAGYQTI

    conjugated to KLH, corresponding to C terminal amino acids 405-416 of Human LAMP1.

  • ポジティブ・コントロール
    • Human Hela cells, rat NRK cells and mouse 3T3 cells.
  • 特記事項

    This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5,268,486 and related patents. Cy and CyDye are trademarks of GE Healthcare Limited.

    Molar Ratio (F/P): 3-9.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab67283 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 1 - 2 µg/ml.

ターゲット情報

  • 機能
    Presents carbohydrate ligands to selectins. Also implicated in tumor cell metastasis.
  • 配列類似性
    Belongs to the LAMP family.
  • 翻訳後修飾
    O- and N-glycosylated; some of the 18 N-linked glycans are polylactosaminoglycans.
  • 細胞内局在
    Cell membrane. Endosome membrane. Lysosome membrane. This protein shuttles between lysosomes, endosomes, and the plasma membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CD107 antigen like family member A antibody
    • CD107 antigen-like family member A antibody
    • CD107a antibody
    • CD107a antigen antibody
    • LAMP 1 antibody
    • LAMP-1 antibody
    • LAMP1 antibody
    • LAMP1_HUMAN antibody
    • LAMPA antibody
    • LGP120 antibody
    • lgpA antibody
    • Lysosomal membrane glycoprotein 120KD antibody
    • Lysosomal Associated Membrane Protein 1 antibody
    • Lysosome associated membrane glycoprotein 1 antibody
    • Lysosome-associated membrane glycoprotein 1 antibody
    • Lysosome-associated membrane protein 1 antibody
    • OTTHUMP00000040663 antibody
    see all

画像

  • Ab67283 staining LAMP1 in HeLa cells by ICC/IF (Immunocytochemistry/Immunofluorescence). Cells were fixed with 4% paraformaldehyde and permeabilized with 0.4% saponin.  Rabbit Anti-PMP70, Atto 488 Conjugate (peroxisomes stained in green) and 2 μg/mL Rabbit Anti-LAMP1, Cy3 Conjugate (ab67283) (lysosomes stained in red). Cells were counterstained with DAPI (blue) to stain nuclei.

  • Ab67283 (2μg) staining LAMP1 in NIH-3T3 cells by ICC/IF (Immunocytochemistry/Immunofluorescence). Cells were fixed with 4% paraformaldehyde. 

参考文献

This product has been referenced in:
  • Li D  et al. An Ebola Virus-Like Particle-Based Reporter System Enables Evaluation of Antiviral Drugs In Vivo under Non-Biosafety Level 4 Conditions. J Virol 90:8720-8 (2016). Read more (PubMed: 27440895) »
  • Heinonen MT  et al. Tubulin- and actin-associating GIMAP4 is required for IFN-? secretion during Th cell differentiation. Immunol Cell Biol 93:158-66 (2015). IF . Read more (PubMed: 25287446) »

See all 3 Publications for this product

レビューと Q&A

Application
Immunocytochemistry/ Immunofluorescence
Sample
Chicken Cell (Cultured Retinal Amacrine cells)
Permeabilization
Yes - Saponin
Specification
Cultured Retinal Amacrine cells
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 4°C
Fixative
Paraformaldehyde
Username

Dr. Wesley Maddox

Verified customer

投稿 Aug 04 2015



I am happy to inform you that this has now been added to our catalogue: Anti-LAMP1 antibody (ab187185)

Thank you for your message. I apologize for the delay of mine! 1) There are no particular requirements for antibodies to work in flow cytometry, so the mentioned antibodies should give positive results using this technique. It is more important to o...

Read More

Thank you very much for your message. As I myself have been in the very same position, I very well understand that you and your supervisor hesitate to test our different CD107a antibodies. At that time I had the permission by my supervisor to test v...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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