製品の概要

  • 製品名Anti-LAMP1 antibody
    LAMP1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to LAMP1
  • アプリケーション適用あり: IHC-P, ICC/IFmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat
  • 免疫原

    Synthetic peptide:

    C-KRSHAGYQTI

    , corresponding to amino acids 407-416 of Human LAMP1

  • ポジティブ・コントロール
    • HeLa cells. IHC-P: FFPE human lung tissue sections.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab19294 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P
ICC/IF
  • 追加情報ICC/IF: Use at a concentration of 5 µg/ml.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能Presents carbohydrate ligands to selectins. Also implicated in tumor cell metastasis.
    • 配列類似性Belongs to the LAMP family.
    • 翻訳後修飾O- and N-glycosylated; some of the 18 N-linked glycans are polylactosaminoglycans.
    • 細胞内局在Cell membrane. Endosome membrane. Lysosome membrane. This protein shuttles between lysosomes, endosomes, and the plasma membrane.
    • Information by UniProt
    • 参照データベース
    • 別名
      • CD107 antigen like family member A antibody
      • CD107 antigen-like family member A antibody
      • CD107a antibody
      • CD107a antigen antibody
      • LAMP 1 antibody
      • LAMP-1 antibody
      • LAMP1 antibody
      • LAMP1_HUMAN antibody
      • LAMPA antibody
      • LGP120 antibody
      • lgpA antibody
      • Lysosomal membrane glycoprotein 120KD antibody
      • Lysosomal Associated Membrane Protein 1 antibody
      • Lysosome associated membrane glycoprotein 1 antibody
      • Lysosome-associated membrane glycoprotein 1 antibody
      • Lysosome-associated membrane protein 1 antibody
      • OTTHUMP00000040663 antibody
      see all

    Anti-LAMP1 antibody 画像

    • IHC image of LAMP1 staining in human lung formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab19294, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    • ab19294 has been successfully used in immunofluorescence procedures. ab19294 shows perinuclear staining in HeLa cells (used at 5 µg/ml). The staining is shown in red. Nuclear staining is shown in blue.

    Anti-LAMP1 antibody (ab19294) 使用論文

    This product has been referenced in:
    • Wavre-Shapton ST  et al. Photoreceptor phagosome processing defects and disturbed autophagy in retinal pigment epithelium of Cln3?ex1-6 mice modelling juvenile neuronal ceroid lipofuscinosis (Batten disease). Hum Mol Genet 24:7060-74 (2015). Mouse . Read more (PubMed: 26450516) »
    • Deisl C  et al. Sodium/hydrogen exchanger NHA2 is critical for insulin secretion in ß-cells. Proc Natl Acad Sci U S A 110:10004-9 (2013). Read more (PubMed: 23720317) »

    See all 4 Publications for this product

    Product Wall

    Thank you for your e-mail and your enquiry about our LAMP1 antibodies. Please find below the answers to your questions: Question 1: which polyclonal one works better? Sure you want to say your products all work great, but which one works greater? T...

    Read More
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (Monocyte-derived Dendritic Cells)
    Specification Monocyte-derived Dendritic Cells
    Fixative Paraformaldehyde
    Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 0.2%
    Username

    Abcam user community

    Verified customer

    投稿 May 30 2006

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (HeLa epithelial)
    Specification HeLa epithelial
    Fixative Paraformaldehyde
    Blocking step Serum as blocking agent for 20 minute(s) · Concentration: 10%
    Username

    Dr. L Knodler

    Verified customer

    投稿 Mar 22 2006

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"