Anti-Laminin gamma 1 抗体 [A5] - BSA and Azide free (ab80580)

Submit an Abreview Q&A (1)Specific References (4)

製品の概要

  • 製品名
    Anti-Laminin gamma 1 antibody [A5] - BSA and Azide free
    Laminin gamma 1 一次抗体 製品一覧
  • 製品の詳細
    Rat monoclonal [A5] to Laminin gamma 1 - BSA and Azide free
  • 由来種
    Rat
  • 特異性
    This antibody uniformly stains all human and murine basement membranes.
  • アプリケーション
    適用あり: IP, IHC-P, IHC-Fr, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Murine EHS laminin preparation.

  • ポジティブ･コントロール
    • LS174T cells. Normal colon or colon carcinoma.
  • 特記事項

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. 

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab80580 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IP
IHC-P
IHC-Fr
ICC/IF
  • 追加情報
    ICC/IF: Use at an assay dependent dilution.
    IHC-P: Use at a concentration of 2 µg/ml.
    IHC Acetone-fixed Fr: Use at a concentration of 2 µg/ml.
    IP: Use at 2µg/mg of lysate. (Native and denatured)

    Staining of acid-alcohol fixed tissues requires antigen unmasking with 15,000U/ml of bovine testicular hyaluronidase in PBS, pH 7.4 for 30 min at 37°C.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • ターゲット情報

    • 機能
      Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.
    • 組織特異性
      Found in the basement membranes (major component).
    • 配列類似性
      Contains 11 laminin EGF-like domains.
      Contains 1 laminin IV type A domain.
      Contains 1 laminin N-terminal domain.
    • ドメイン
      The alpha-helical domains I and II are thought to interact with other laminin chains to form a coiled coil structure.
      Domains VI and IV are globular.
    • 細胞内局在
      Secreted > extracellular space > extracellular matrix > basement membrane.
    • Information by UniProt
    • 参照データベース
      see all
    • 別名
      • LAMB2 antibody
      • lamc1 antibody
      • LAMC1_HUMAN antibody
      • Laminin B2 chain antibody
      • Laminin subunit gamma-1 antibody
      • Laminin, gamma 1 (formerly LAMB2) antibody
      • Laminin-1 subunit gamma antibody
      • Laminin-10 subunit gamma antibody
      • Laminin-11 subunit gamma antibody
      • Laminin-2 subunit gamma antibody
      • Laminin-3 subunit gamma antibody
      • Laminin-4 subunit gamma antibody
      • Laminin-6 subunit gamma antibody
      • Laminin-7 subunit gamma antibody
      • Laminin-8 subunit gamma antibody
      • Laminin-9 subunit gamma antibody
      • MGC87297 antibody
      • S-LAM gamma antibody
      • S-laminin subunit gamma antibody
      see all

    画像

    • Immunohistochemistry (Frozen sections) - Anti-Laminin gamma 1 [A5] antibody - BSA and Azide free (ab80580)
      Immunohistochemistry (Frozen sections) - Anti-Laminin gamma 1 [A5] antibody - BSA and Azide free (ab80580)
      Frozen human tonsil stained with ab80580 at 2µg/ml using peroxidase-conjugate and DAB chromogen. Note basement membrane and cell surface staining of blood vessels.
    • Immunoprecipitation - Anti-Laminin gamma 1 [A5] antibody - BSA and Azide free (ab80580)
      Immunoprecipitation - Anti-Laminin gamma 1 [A5] antibody - BSA and Azide free (ab80580)
      Native IP using LS174T cells:
      IP 2µg/mg lysate.
      WB at 2µg/ml.

    参考文献

    This product has been referenced in:
    • Calyjur PC  et al. The mdx Mutation in the 129/Sv Background Results in a Milder Phenotype: Transcriptome Comparative Analysis Searching for the Protective Factors. PLoS One 11:e0150748 (2016). IHC . Read more (PubMed: 26954670) »
    • Ljubimov AV  et al. Human corneal basement membrane heterogeneity: topographical differences in the expression of type IV collagen and laminin isoforms. Lab Invest 72:461-73 (1995). ICC/IF, IHC-P, IHC-Fr, IP ; Human, Mouse . Read more (PubMed: 7723285) »

    See all 4 Publications for this product

    ab80580 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    レビューと Q&A

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    1 Review or Q&A

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    Hi, Could you please let me know which secondary antibodies were used to obtain the images on the product sheet? Thanks,

    Thank you for contacting us. We can recommend using antibody ab106783, ab99667, ab99666 etc. Rabbit or goat anti rat IgG also cross reacts with IgG2a so anti rat IgGs can also be used. I hope this information is helpful to you. Please do not hesitate...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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