製品の概要

  • 製品名
    Anti-Laminin beta 1 antibody [LT3]
    Laminin beta 1 一次抗体 製品一覧
  • 製品の詳細
    Rat monoclonal [LT3] to Laminin beta 1
  • アプリケーション
    適用あり: ICC/IF, WB, IP, IHC-Fr, IHC (Methanol fixed), IHC-Pmore details
  • 種交差性
    交差種: Mouse, Hamster, Human, Pig
  • 免疫原

    Murine EHS laminin preparation.

  • ポジティブ・コントロール
    • LS174T cells. Normal colon or colon carcinoma.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab44941 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use at an assay dependent dilution.
WB 1/200. Detects a band of approximately 210 kDa.
IP 1/400.
IHC-Fr 1/400. Use with acetone fixed tissues.
IHC (Methanol fixed) 1/400.
IHC-P Use at an assay dependent dilution.

ターゲット情報

  • 機能
    Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.
  • 配列類似性
    Contains 13 laminin EGF-like domains.
    Contains 1 laminin IV type B domain.
    Contains 1 laminin N-terminal domain.
  • ドメイン
    The alpha-helical domains I and II are thought to interact with other laminin chains to form a coiled coil structure.
    Domains VI and IV are globular.
  • 細胞内局在
    Secreted > extracellular space > extracellular matrix > basement membrane. Major component.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CLM antibody
    • Cutis laxa with marfanoid phenotype antibody
    • LAM B1 antibody
    • LAMB 1 antibody
    • LAMB1 antibody
    • LAMB1_HUMAN antibody
    • Laminin B1 antibody
    • Laminin B1 chain antibody
    • Laminin beta 1 chain antibody
    • Laminin beta 1 chain precursor antibody
    • Laminin beta1 antibody
    • Laminin subunit beta 1 antibody
    • Laminin subunit beta-1 antibody
    • Laminin-1 subunit beta antibody
    • Laminin-10 subunit beta antibody
    • Laminin-12 subunit beta antibody
    • Laminin-2 subunit beta antibody
    • Laminin-6 subunit beta antibody
    • Laminin-8 subunit beta antibody
    • LIS5 antibody
    • MGC142015 antibody
    see all

画像

  • ICC/IF image of ab44941 staining human prostate cells.  The cells were fixed with 4% paraformaldehyde, permeabilised with 0.8% Triton X-100, and blocked with 20% serum for 1 hour at 24°C.  The primary anitibody was diluted 1/400 in in 20% horse serum in PBS and incubated for 20 minutes at 4°C.  An Alexa Fluor® 488 conjugated donkey anti-rat was used as the secondary antibody.

    The blue staining is the cell nuclei, the green staining shows the laminin beta 1.

    See Abreview

  • ab44941 staining Laminin beta 1 in porcine jejunum tissue by Immunohistochemistry (Frozen sections).
    Tissue was fixed in methanol/acetone, permeabilized using 0.3% Triton, blocked with 1% normal goat serum for 10 minutes at room temperature and then incubated with ab44941 at a 1/800 dilution for 2 hours. The secondary used was an Alexa-Fluor 488 conjugated goat anti-rat IgG (H+L) used at a 1/200 dilution.

    See Abreview

参考文献

This product has been referenced in:
  • Yuan X  et al. Histone acetylation is involved in TCDD-induced cleft palate formation in fetal mice. Mol Med Rep 14:1139-45 (2016). Read more (PubMed: 27279340) »
  • Petley-Ragan LM  et al. Accumulation of Laminin Monomers in Drosophila Glia Leads to Glial Endoplasmic Reticulum Stress and Disrupted Larval Locomotion. J Neurosci 36:1151-64 (2016). Read more (PubMed: 26818504) »

See all 4 Publications for this product

レビューと Q&A

Application
Western blot
Sample
Mouse Cell lysate - whole cell (Melanoma cell lines)
Gel Running Conditions
Reduced Denaturing
Loading amount
40 µg
Specification
Melanoma cell lines
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Mar 24 2017

Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (melanoma cell line)
Total protein in input
1000 µg
Immuno-precipitation step
Protein G
Specification
melanoma cell line
Username

Abcam user community

Verified customer

投稿 Nov 18 2015

Thank you for that information.

I have gone ahead and set up a credit for the cost of your original order. Once we recieve the 3 vials of unopened antibody from you the credit will be applied.

When you return the goods, please ensur...

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Thank you for your reply.

Can you please let me know how the unused units of the antibody were stored? If they have been stored at 4C since you received them, I can go ahead and credit the cost of the order back to you and also organize for t...

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Thank you for your reply and for providing that information.

I just had a few more follow up questions:

1 - You received 4 vials of ab44941. How many of the bottles did you test? I ask incase the issue may be limited to a specific b...

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Thank you for contacting Abcam.

I am sorry that you are experiencing some issues with ab44941. The antibody is covered under our Abpromise for six months and is guaranteed to work in IHC-P on Mouse, Hamster, Human & Pig samples . If we ca...

Read More

Thank you for your inquiry.


When conducting IHC or IF, it is generally not advisable to use antibodies which are raised in the same species as the target sample (e.g. a mouse monoclonal antibody against mouse samples). The secondary a...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Pig Tissue sections (small intestine (jejunum))
Specification
small intestine (jejunum)
Fixative
Methanol/Acetone
Permeabilization
Yes - 0.3% Triton
Blocking step
(agent) for 10 minute(s) · Concentration: 1% · Temperature: RT°C
Username

Abcam user community

Verified customer

投稿 Mar 02 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (prostate)
Specification
prostate
Fixative
Formaldehyde
Permeabilization
Yes - 0.2% Triton-X
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Username

Abcam user community

Verified customer

投稿 Oct 14 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Prostate)
Specification
Prostate
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.8% Triton X-100
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 20% · Temperature: 24°C
Username

Abcam user community

Verified customer

投稿 Jan 07 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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