Anti-Laminin beta 1 抗体 [EPR3190(2)] (ab109293)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3190(2)] to Laminin beta 1
- Suitable for: Flow Cyt (Intra), WB
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Laminin beta 1 antibody [EPR3190(2)]
Laminin beta 1 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR3190(2)] to Laminin beta 1 -
由来種
Rabbit -
アプリケーション
適用あり: Flow Cyt (Intra), WBmore details
適用なし: ICC/IF,IHC-P or IP -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- WB: Human fetal kidney, HeLa and A431 lysates. Flow Cyt (intra): A431 cells
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR3190(2) -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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KO cell lines
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KO cell lysates
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab109293の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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Flow Cyt (Intra) |
1/100 - 1/500.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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WB |
1/1000 - 1/10000. Detects a band of approximately 220 kDa (predicted molecular weight: 198 kDa).
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特記事項 |
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Flow Cyt (Intra)
1/100 - 1/500. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/1000 - 1/10000. Detects a band of approximately 220 kDa (predicted molecular weight: 198 kDa). |
ターゲット情報
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機能
Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. -
配列類似性
Contains 13 laminin EGF-like domains.
Contains 1 laminin IV type B domain.
Contains 1 laminin N-terminal domain. -
ドメイン
The alpha-helical domains I and II are thought to interact with other laminin chains to form a coiled coil structure.
Domains VI and IV are globular. -
細胞内局在
Secreted > extracellular space > extracellular matrix > basement membrane. Major component. - Information by UniProt
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参照データベース
- Entrez Gene: 3912 Human
- Entrez Gene: 16777 Mouse
- Entrez Gene: 298941 Rat
- Omim: 150240 Human
- SwissProt: P07942 Human
- SwissProt: P02469 Mouse
- Unigene: 650585 Human
- Unigene: 172674 Mouse
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別名
- CLM antibody
- Cutis laxa with marfanoid phenotype antibody
- LAM B1 antibody
see all
画像
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All lanes : Anti-Laminin beta 1 antibody [EPR3190(2)] (ab109293) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : LAMB1 knockout HeLa cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 198 kDa
Observed band size: 200 kDa why is the actual band size different from the predicted?Lanes 1- 2: Merged signal (red and green). Green - ab109293 observed at 200 kDa. Red - Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) observed at 50 kDa.
ab109293 was shown to react with Laminin beta 1 in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab265414 (knockout cell lysate ab257499) was used. Wild-type HeLa and LAMB1 knockout HeLa cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab109293 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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Intracellular flow cytometric analysis of permeabilized A431 cells using ab109293 (red) at a diluiton of 1/100 or a rabbit IgG (negative) (green).
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All lanes : Anti-Laminin beta 1 antibody [EPR3190(2)] (ab109293) at 1/1000 dilution
Lane 1 : Human fetal kidney lysate
Lane 2 : A431 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 198 kDa
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab109293 は 2 報の論文で使用されています。
- Xu S et al. Curcumin suppresses intestinal microvascular endothelial cells invasion and angiogenesis induced by activated platelets. Exp Ther Med 18:1099-1106 (2019). PubMed: 31316605
- Weng S et al. Role of quercetin in protecting ARPE-19 cells against H2O2-induced injury via nuclear factor erythroid 2 like 2 pathway activation and endoplasmic reticulum stress inhibition. Mol Med Rep 16:3461-3468 (2017). PubMed: 28713895