製品の概要

  • 製品名Anti-Laminin antibody
    Laminin 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Laminin
  • アプリケーション適用あり: WB, ICC/IF, IHC-Fr, IHC-FoFrmore details
  • 種交差性
    交差種: Mouse, Rat
    交差が予測される動物種: all Mammals
  • 免疫原

    Full length native protein isolated from mouse EHS tumor(purified)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in MEF1 cell line in IF/ICC.

法規制情報

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab30320 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/5000. Predicted molecular weight: 337 kDa.
ICC/IF 1/100 - 1/2500.
IHC-Fr 1/1000.
IHC-FoFr 1/1000.

ターゲット情報

  • 機能Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.
  • 組織特異性Broadly expressed in: skin, heart, lung, and the reproductive tracts.
  • 配列類似性Contains 11 laminin EGF-like domains.
    Contains 1 laminin IV type A domain.
    Contains 1 laminin N-terminal domain.
  • ドメインThe alpha-helical domains I and II are thought to interact with other laminin chains to form a coiled coil structure.
    Domain IV is globular.
  • 細胞内局在Secreted > extracellular space > extracellular matrix > basement membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • LAMA 1 antibody
    • LAMA antibody
    • LAMA1 antibody
    • LAMB1 antibody
    • Lamc3 antibody
    • LAMC3_HUMAN antibody
    • Laminin A chain antibody
    • Laminin alpha 1 antibody
    • Laminin alpha 1 chain antibody
    • Laminin B1 chain antibody
    • Laminin subunit beta 1 antibody
    • Laminin subunit gamma-3 antibody
    • Laminin-12 subunit gamma antibody
    • Laminin-14 subunit gamma antibody
    • Laminin-15 subunit gamma antibody
    see all

Anti-Laminin antibody 画像

  • ICC/IF image of ab30320 stained MEF1 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab30320, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
  • Rat peripheral nerve (low and high magnification; formalin fixed, frozen sections). Immuno-FITC stain (color inverted) with 1:1000 anti-Laminin1 antibody (ab30320).

Anti-Laminin antibody (ab30320) 使用論文

This product has been referenced in:
  • Bruscoli S  et al. Lack of glucocorticoid-induced leucine zipper (GILZ) deregulates B-cell survival and results in B-cell lymphocytosis in mice. Blood 126:1790-801 (2015). WB ; Mouse . Read more (PubMed: 26276664) »
  • Hosaka K  et al. Tumour PDGF-BB expression levels determine dual effects of anti-PDGF drugs on vascular remodelling and metastasis. Nat Commun 4:2129 (2013). Read more (PubMed: 23831851) »

See all 5 Publications for this product

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The antibody is a whole antiserum, without purification. As such, it is a mixture of various proteins, and immunogloblin concentration is not assayed. In general, serum is expected to contain about 10 mg/ml of to...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"