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Purified laminin P1 fragment from human placenta.
This antibody primarily labels basal membranes (especially s. c. lamina lucida) and may be used to detect disturbances in the basal membrane during tumour invasion.
Our Abpromise guarantee covers the use of ab23753 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use a concentration of 5 µg/ml. Can be paired for Sandwich ELISA with Mouse monoclonal [LAM-89] to Laminin (ab49726). For sandwich ELISA, use this antibody as Capture at 5µg/ml with ab49726 as Detection.|
|ICC/IF||Use at an assay dependent concentration.|
|IHC-P||1/200 - 1/600.
After de-waxing the tissue slices they are treated with 0.2% hyaluronidase in TBS 15 min at 37°C. There after, non-specific binding is blocked by blocking serum or 3% BSA in TBS. For peroxidase systems blocking with 1% peroxide solution in TBS for 30 min at RT is recommended.
|ELISA||Use at an assay dependent concentration.|
Human skin paraffin section at 1:200 dilution.
ab23753 has not yet been referenced specifically in any publications.