製品の概要

  • 製品名Anti-Laminin antibody
    Laminin 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Laminin
  • 特異性Laminin fragment P1 100%, collagen type I and IV <0.1%, fibronectin <0.1%
  • アプリケーション適用あり: RIA, Sandwich ELISA, ICC/IF, IHC-P, ELISAmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Purified laminin P1 fragment from human placenta.

  • ポジティブ・コントロール
    • Human skin
  • 特記事項


    This antibody primarily labels basal membranes (especially s. c. lamina lucida) and may be used to detect disturbances in the basal membrane during tumour invasion.

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab23753 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
RIA 1/1000.
Sandwich ELISA Use a concentration of 5 µg/ml. For sandwich ELISA, use this antibody as Capture at 5µg/ml with ab49726 as Detection.
ICC/IF Use at an assay dependent concentration.
IHC-P 1/200 - 1/600.

After de-waxing the tissue slices they are treated with 0.2% hyaluronidase in TBS 15 min at 37°C. There after, non-specific binding is blocked by blocking serum or 3% BSA in TBS. For peroxidase systems blocking with 1% peroxide solution in TBS for 30 min at RT is recommended.

ELISA Use at an assay dependent concentration.

ターゲット情報

  • 機能Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.
  • 組織特異性Broadly expressed in: skin, heart, lung, and the reproductive tracts.
  • 配列類似性Contains 11 laminin EGF-like domains.
    Contains 1 laminin IV type A domain.
    Contains 1 laminin N-terminal domain.
  • ドメインThe alpha-helical domains I and II are thought to interact with other laminin chains to form a coiled coil structure.
    Domain IV is globular.
  • 細胞内局在Secreted > extracellular space > extracellular matrix > basement membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • LAMA 1 antibody
    • LAMA antibody
    • LAMA1 antibody
    • LAMB1 antibody
    • Lamc3 antibody
    • LAMC3_HUMAN antibody
    • Laminin A chain antibody
    • Laminin alpha 1 antibody
    • Laminin alpha 1 chain antibody
    • Laminin B1 chain antibody
    • Laminin subunit beta 1 antibody
    • Laminin subunit gamma-3 antibody
    • Laminin-12 subunit gamma antibody
    • Laminin-14 subunit gamma antibody
    • Laminin-15 subunit gamma antibody
    see all

Anti-Laminin antibody 画像

プロトコール

Anti-Laminin antibody (ab23753) 使用論文

ab23753 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting us.

This product has PBS buffer without addition of BSA and preservatives.


I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information. <...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"