Anti-Laminin alpha 5 抗体 [4C7] (ab17107)

製品の概要

  • 製品名Anti-Laminin alpha 5 antibody [4C7]
    Laminin alpha 5 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [4C7] to Laminin alpha 5
  • 特異性This antibody was originally thought to recognize laminin alpha 1 or A, but further characterization has confirmed its specificity for the laminin alpha 5 chain [see Tiger, C.F. (1997) J. Biol. Chem. 272: 28590].
  • アプリケーション適用あり: IHC-Pmore details
  • 種交差性
    交差種: Human
    非交差種: Mouse, Rat
  • 免疫原

    Full length native protein (purified) corresponding to Human Laminin alpha 5.
    Database link: O15230

  • ポジティブ・コントロール
    • Skin
  • 特記事項


    This antibody reacts with the terminal globular domain of the A chain. The staining with this antibody is sensitive to fixation of paraffin tissues and use of proteolytic enzymatic treatment.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab17107 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/5 - 1/20. Perform enzymatic antigen retrieval before commencing with IHC staining protocol.

ターゲット情報

  • 関連性Laminins are a family of heterotrimeric extracellular glycoproteins consisting of alpha, beta, and gamma chains. Laminins are thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. Laminin alpha 5 (LAMA5) belongs to the alpha subfamily of laminin chains and is a major component of basement membranes. Laminin alpha 5 is the alpha chain in the Laminin 15 complex which is a heterotrimer composed of alpha 5/beta 2/gamma 3 which are bound to each other by disulfide bonds into a cross shaped molecule comprising one long and three short arms with globules at each end.
  • 細胞内局在Secreted; basement membrane.
  • 参照データベース
  • 別名
    • KIAA0533 antibody
    • KIAA1907 antibody
    • LAMA5 antibody
    • Laminin alpha 5 chain antibody
    • Laminin subunit alpha 5 antibody
    • Laminin subunit alpha-5 antibody
    • Laminin, alpha 5 antibody
    • Laminin-10 subunit alpha antibody
    • Laminin-11 subunit alpha antibody
    • Laminin-15 subunit alpha antibody
    • RP11-157P1.6 antibody
    see all

Anti-Laminin alpha 5 antibody [4C7] 画像

  • ab17107 at 1/25 staining human colon tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step was performed prior to incubation with the antibody for 16 hours. An HRP conjugated goat anti-mouse antibody was used as the secondary.

    See Abreview

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human renal carcinoma tissue, staining Laminin alpha 5 with ab17107.

Anti-Laminin alpha 5 antibody [4C7] (ab17107) 使用論文

This product has been referenced in:
  • Mackey AL  et al. Sequenced response of extracellular matrix deadhesion and fibrotic regulators after muscle damage is involved in protection against future injury in human skeletal muscle. FASEB J 25:1943-59 (2011). IHC-Fr ; Human . Read more (PubMed: 21368102) »

See 1 Publication for this product

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Thank you for your email. I am sorry to hear that you have been experiencing problems with this antibody.

The antibody ab5654 is not tested in IHC-P application so Abpromise guarantee will not apply to this product. This antibody was however t...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (colon)
Specification colon
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Antigen Unmasking Solution, Citric Acid Based (Vector Lab)
Permeabilization No
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
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Abcam user community

Verified customer

投稿 Jul 03 2007

Thank you for your email. Our source for this antibody has kindly provided me with the following information. In the study by Engvall, 1990, it is stated: "4C7, binds to the globular domain at the end of the long arm (Engvall et al., 1986). This domain...

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This is the only information available to us regarding the immunogen and epitope. Further details can be found in the following two publications: Engvall E et al. Distribution and isolation of four laminin variants; tissue restricted distribution ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"