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Our Abpromise guarantee covers the use of ab55409 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 1 µg/ml.|
|WB||Use a concentration of 1 - 5 µg/ml.
This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.
ab55409 staining Laminin 2 alpha in human placenta tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with H2O2 for 10 minutes at 25°C; antigen retrieval was by heat mediation in citrate pH 6 at 100°C for 20 minutes. Samples were incubated with primary antibody (1/500) for 20 minutes at 25°C. An undiluted HRP polymer-conjugated goat anti-mouse IgG polyclonal was used as the secondary antibody.
ab55409 has not yet been referenced specifically in any publications.