製品の概要

  • 製品名Anti-Laminin 2 alpha antibody [4H8-2]
    Laminin 2 alpha 一次抗体 製品一覧
  • 製品の詳細
    Rat monoclonal [4H8-2] to Laminin 2 alpha
  • アプリケーション適用あり: IHC-Fr, ICC/IF, WB, ELISA, ICC, IPmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Heart Laminin 2 (Mouse).

  • エピトープThe epitope recognized by the antibody resides in the N-terminal portion of the alpha 2 chain of laminin.
  • ポジティブ・コントロール
    • Human tongue.
  • 特記事項Storage in frost-free freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab11576 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-Fr Use a concentration of 4 - 8 µg/ml.

This concentration was determined for acetone-fixed frozen sections of human tongue.

ICC/IF Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Detects a band of approximately 400 kDa.
ELISA Use at an assay dependent concentration.
ICC Use at an assay dependent concentration.
IP Use at an assay dependent concentration.

ターゲット情報

  • 機能Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.
  • 組織特異性Placenta, striated muscle, peripheral nerve, cardiac muscle, pancreas, lung, spleen, kidney, adrenal gland, skin, testis, meninges, choroid plexus, and some other regions of the brain; not in liver, thymus and bone.
  • 関連疾患Defects in LAMA2 are the cause of merosin-deficient congenital muscular dystrophy type 1A (MDC1A) [MIM:607855]. MDC1A is characterized by difficulty walking, hypotonia, proximal weakness, hyporeflexia, and white matter hypodensity on MRI.
  • 配列類似性Contains 17 laminin EGF-like domains.
    Contains 5 laminin G-like domains.
    Contains 2 laminin IV type A domains.
    Contains 1 laminin N-terminal domain.
  • ドメインThe alpha-helical domains I and II are thought to interact with other laminin chains to form a coiled coil structure.
    Domains VI, IV and G are globular.
  • 細胞内局在Secreted > extracellular space > extracellular matrix > basement membrane. Major component.
  • Information by UniProt
  • 参照データベース
  • 別名
    • LAMA 2 antibody
    • LAMA2 antibody
    • LAMA2_HUMAN antibody
    • Laminin alpha 2 (merosin congenital muscular dystrophy) antibody
    • Laminin alpha 2 antibody
    • Laminin alpha 2 chain antibody
    • Laminin alpha 2 subunit antibody
    • Laminin M antibody
    • Laminin M chain antibody
    • Laminin subunit alpha-2 antibody
    • Laminin-12 subunit alpha antibody
    • Laminin-2 subunit alpha antibody
    • Laminin-4 subunit alpha antibody
    • LAMM antibody
    • Merosin heavy chain antibody
    see all

Anti-Laminin 2 alpha antibody [4H8-2] 画像

  • ab11576 staining C57B6 mouse skeletal muscle cryostate tissue sections by IHC-Fr.  The section was acetone fixed and blocked with 5% BSA for 30 mintes at RT, prior to incubation with the primary antibody (diluted 1/200 in blocking buffer) for 2 hours.  A rhodamine-conjugated goat anti-rat antibody was used as the secondary.

    See Abreview

Anti-Laminin 2 alpha antibody [4H8-2] (ab11576) 使用論文

This product has been referenced in:
  • Rodgers JT  et al. mTORC1 controls the adaptive transition of quiescent stem cells from G0 to G(Alert). Nature 509:393-6 (2014). Mouse . Read more (PubMed: 24870234) »
  • Park R  et al. Aß1-42 reduces P-glycoprotein in the blood-brain barrier through RAGE-NF-?B signaling. Cell Death Dis 5:e1299 (2014). Read more (PubMed: 24967961) »

See all 13 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Human Tissue sections (muscle)
Permeabilization Yes - PBS triton0.5%
Specification muscle
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
Fixative Formaldehyde
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投稿 Mar 14 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (C57B6 mouse skeletal muscle cryostate sections)
Specification C57B6 mouse skeletal muscle cryostate sections
Fixative Acetone
Permeabilization No
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
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Verified customer

投稿 Feb 13 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"