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Full length native protein (purified) (Mouse EHS tumour).
Laminin antibodies are widely used to label blood vessels and basement membranes.
Our Abpromise guarantee covers the use of ab7463 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-Fr||1/500 - 1/2000.|
|Inhibition Assay||Use at an assay dependent concentration. This product functionally inhibits laminin in mouse and rat. Not tested in human tissue.|
|IHC-P||1/500 - 1/2000. Perform enzymatic antigen retrieval before commencing with IHC staining protocol.
Immunostaining enhanced by antigen retrieval with pepsin - apply 100ul of 4mg/ml pepsin dissolved in 0.01M HCl for 1 hour at 37C, especially paraffin tissue.
|WB||1/100 - 1/5000.
Use 5-7% of gel for electrophoresis.
|ICC/IF||1/50 - 1/200.|
|IHC-FrFl||Use at an assay dependent concentration. PubMed: 25077029|
Ab7463 staining Laminin 1+2 in HeLa cells by ICC/IF (Immunocytochemistry/Immunofluorescence). Samples were incubated with primary antibody at 1:50 dilution. Hoechst 33258 (blue) was used as a nuclear counterstain.
Ab7463 staining Lamin 1+2 in HeLa cells by ICC/IF (Immunocytochemistry/Immunofluorescence). Samples were incubated with primary antibody at 1:5 dilution. An Alexa Fluor® 488- conjugated Goat to rabbit IgG was used as a secondary antibody. DAPI was used as a nuclear stain and an Alexa Fluor® 568 phalloidin (red) was used to stain actin fllaments.
ab7463 staining Laminin 1+2 in human MDA-MB-231 by ICC/IF (Immunocytochemistry/ Immunofluorescence). The cells were paraformaldehyde fixed, permeabilized in 0.1% Triton X-100 buffer and blocked in 5% serum for 30 minutes at 25°C. The primary antibody was diluted 1/250 and incubated with sample for 1 hour at 37°C. A Alexa Fluor® 488 conjugated goat polyclonal to rabbit IgG was used undiluted, as secondary.