製品の概要

  • 製品名
    Anti-Laminin 1+2 antibody
  • 製品の詳細
    Rabbit polyclonal to Laminin 1+2
  • 特異性
    This antibody is pan-specific and reacts well with all laminin isoforms tested: laminin-1 (alpha-1, beta-1, and gamma-1) and laminin-2 (alpha-2, beta-1, and gamma-1).
  • アプリケーション
    適用あり: IHC-Fr, Inhibition Assay, IHC-P, WB, ICC/IF, IHC-FrFlmore details
  • 種交差性
    交差種: Mouse, Rat, Sheep, Rabbit, Human
  • 免疫原

    Full length native protein (purified) (Mouse EHS tumour).

  • ポジティブ・コントロール
    • Basement membranes (blood vessels of any tissue, tumors and nerve).
  • 特記事項


    Laminin antibodies are widely used to label blood vessels and basement membranes.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー
    Preservative: 0.05% Sodium azide
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 一次抗体 備考
    Laminin antibodies are widely used to label blood vessels and basement membranes.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab7463 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-Fr 1/500 - 1/2000.
Inhibition Assay Use at an assay dependent concentration. This product functionally inhibits laminin in mouse and rat. Not tested in human tissue.
IHC-P 1/500 - 1/2000. Perform enzymatic antigen retrieval before commencing with IHC staining protocol.

Immunostaining enhanced by antigen retrieval with pepsin - apply 100ul of 4mg/ml pepsin dissolved in 0.01M HCl for 1 hour at 37C,  especially paraffin tissue.

WB 1/100 - 1/5000.

Use 5-7% of gel for electrophoresis.

ICC/IF 1/50 - 1/200.
IHC-FrFl Use at an assay dependent concentration. PubMed: 25077029

ターゲット情報

画像

  • Ab7463 staining Laminin 1+2 in HeLa cells by ICC/IF (Immunocytochemistry/Immunofluorescence). Samples were incubated with primary antibody at 1:50 dilution. Hoechst 33258 (blue) was used as a nuclear counterstain. 

  • Ab7463 staining Lamin 1+2 in HeLa cells by ICC/IF (Immunocytochemistry/Immunofluorescence). Samples were incubated with primary antibody at 1:5 dilution. An Alexa Fluor® 488- conjugated Goat to rabbit IgG was used as a secondary antibody. DAPI was used as a nuclear stain and an Alexa Fluor® 568 phalloidin (red) was used to stain actin fllaments. 

  • All lanes : Anti-Laminin 1+2 antibody (ab7463) at 1 µg/ml

    Lane 1 : EHS
    Lane 2 : Heart


    Observed band size : 200,400 kDa (why is the actual band size different from the predicted?)
  • Ab7463 staining Laminin in rat spinal cord and dorsal root by immunohistochemistry using paraformaldehyde/paraffin embedded tissue. Pepsin antigen retrieval was performed on this tissue sample.Ab7463 staining Laminin in rat spinal cord and dorsal root by immunohistochemistry using paraformaldehyde/paraffin embedded tissue. Pepsin antigen retrieval was performed on this tissue sample.
  • ab7463 staining Laminin 1+2 in human MDA-MB-231 by ICC/IF (Immunocytochemistry/ Immunofluorescence). The cells were paraformaldehyde fixed, permeabilized in 0.1% Triton X-100 buffer and blocked in 5% serum for 30 minutes at 25°C. The primary antibody was diluted 1/250 and incubated with sample for 1 hour at 37°C. A Alexa Fluor® 488 conjugated goat polyclonal to rabbit IgG was used undiluted, as secondary.

参考文献

This product has been referenced in:
  • Mao M  et al. Genetic dissection of anterior segment dysgenesis caused by a Col4a1 mutation in mouse. Dis Model Mech 10:475-485 (2017). Read more (PubMed: 28237965) »
  • Espitia Pinzon N  et al. Astrocyte-derived tissue Transglutaminase affects fibronectin deposition, but not aggregation, during cuprizone-induced demyelination. Sci Rep 7:40995 (2017). Read more (PubMed: 28128219) »

See all 16 Publications for this product

レビューと Q&A

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry free floating
Sample
Mouse Tissue sections (Breast tissue)
Specification
Breast tissue
Username

Abcam user community

Verified customer

投稿 Feb 17 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (MDA-MB-231)
Specification
MDA-MB-231
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% Triton X-100
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 May 06 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (normal brain)
Specification
normal brain
Fixative
Paraformaldehyde
Antigen retrieval step
None
Permeabilization
No
Blocking step
TNB Blocking Reagent (from another company) as blocking agent for 30 minute(s) · Concentration: 0.5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Jul 28 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (Brain)
Specification
Brain
Fixative
Acetone
Permeabilization
No
Blocking step
TNB Blocking Reagent (from another company) as blocking agent for 30 minute(s) · Concentration: 0.5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Jul 28 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (intestinal mucosa)
Loading amount
70 µg
Specification
intestinal mucosa
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Dr. Sofia Tedelind

Verified customer

投稿 Feb 11 2008

Thank you for your enquiry. This antibody will reavt with beta 1 and gamma 1 and reacts with mouse. I am not sure that I understand your question about the immunofluorescence though. This antibody has been tested for use in staining of frozen and paraf...

Read More

I was informed that unfortunately, we do not know the protein concentration. For practical purposes, I would recommend using the isotype control at a concentration in the range of 1 mg/ml. I'm sorry I couldn't help you more on this occasion,

Unfortunately, we do not know what chain the antibody is directed against specifically. If you have any more questions, please contact us again.

The following should be helpful: The fixation is routine paraformaldehyde or formalin fixation of tissue prior to paraffin embedding. However, the staining procedure must include an antigen retrieval step pretreating the deparaffinized sections wit...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

登録