製品の概要

  • 製品名Anti-Lamin B1 antibody
    Lamin B1 一次抗体 製品一覧
  • 製品の詳細
    Chicken polyclonal to Lamin B1
  • アプリケーション適用あり: ICC/IF, WBmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat, Chicken, Cow, Zebrafish
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 400 - 500 of Human Lamin B1.

    (Peptide available as ab16375.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in the following human whole cell lysates: HeLa; Jurkat; A431; NIH3T3.

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab90169 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 73 kDa (predicted molecular weight: 66 kDa).

ターゲット情報

  • 機能Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin.
  • 関連疾患Defects in LMNB1 are the cause of leukodystrophy demyelinating autosomal dominant adult-onset (ADLD) [MIM:169500]. ADLD is a slowly progressive and fatal demyelinating leukodystrophy, presenting in the fourth or fifth decade of life. Clinically characterized by early autonomic abnormalities, pyramidal and cerebellar dysfunction, and symmetric demyelination of the CNS. It differs from multiple sclerosis and other demyelinating disorders in that neuropathology shows preservation of oligodendroglia in the presence of subtotal demyelination and lack of astrogliosis.
  • 配列類似性Belongs to the intermediate filament family.
  • 翻訳後修飾B-type lamins undergo a series of modifications, such as farnesylation and phosphorylation. Increased phosphorylation of the lamins occurs before envelope disintegration and probably plays a role in regulating lamin associations.
  • 細胞内局在Nucleus inner membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • ADLD antibody
    • lamin B1 antibody
    • Lamin-B1 antibody
    • LMN antibody
    • LMN2 antibody
    • LMNB antibody
    • Lmnb1 antibody
    • LMNB1_HUMAN antibody
    • MGC111419 antibody
    • OTTHUMP00000159218 antibody
    see all

Anti-Lamin B1 antibody 画像



  • Predicted band size : 66 kDa

    Lanes 1, 3 and 5: Wild-type HAP1 nuclear lysate (20 µg)
    Lanes 2, 4 and 6: Lamin B1 knockout HAP1 nuclear lysate (20 µg)
    Lanes 1 and 2: Green signal from target - ab90169 observed at 75 kDa
    Lanes 3 and 4: Red signal from loading control - ab10799 observed at 18 kDa
    Lanes 5 and 6: Merged (red and green) signal
    ab90169 was shown to specifically react with lamin B1 when lamin B1 knockout samples were used (in which a mutation has induced an N-terminal truncation of lamin B1). Wild-type and lamin B1 knockout samples were subjected to SDS-PAGE. ab90169 and ab10799 (loading control to histone H3) were both diluted at 1/1000 and incubated overnight at 4°C. Blots were developed with goat anti-rabbit IgG (H + L) and goat anti-mouse IgG (H + L) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

  • All lanes : Anti-Lamin B1 antibody (ab90169) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat polyclonal to Chicken IgY - H&L (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 66 kDa
    Observed band size : 73 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 28 kDa,37 kDa,50 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 4 minutes
  • ICC/IF image of ab90169 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab90169 at 5ug overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti- chicken IgY (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Anti-Lamin B1 antibody (ab90169) at 1 µg/ml + NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 10 µg

    Secondary
    Goat polyclonal Secondary Antibody to Chicken IgY - H&L (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 66 kDa
    Observed band size : 75 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 18 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 20 minutes

Anti-Lamin B1 antibody (ab90169) 使用論文

ab90169 has not yet been referenced specifically in any publications.

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