Anti-Lambda Light chain 抗体 (HRP) (ab9007)

製品の概要

  • 製品名
    Anti-Lambda Light chain antibody (HRP)
    Lambda Light chain 一次抗体 製品一覧
  • 製品の詳細
    Sheep polyclonal to Lambda Light chain (HRP)
  • 標識
    HRP
  • 特異性
    The unconjugated immunoglobulin gives a single arc when tested by IEP against human serum. Identity has been confirmed by double diffusion (Ouchterlony) against human serum and an anti- HumanLambda of known specificity.
  • アプリケーション
    適用あり: IHC-Fr, ELISA, WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    The immunogen used was human lambda-chain, purified from disease state urines and shown to be homogeneous by SDS-PAGE.

  • 特記事項
    The following points should be observed when using this product: 1) Dilutions of the conjugate must be made in a suitable buffer (e.g. 0.15M Phosphate buffered saline,pH7.2) 2) It is recommended that dilutions of the conjugate are made in polypropylene or polyethylene containers. Other materials (e.g. polystyrene, some grades of glass) may be detrimental to the conjugate. 3) Working dilutions of the conjugate should be prepared immediately prior to use. 4) Peroxidase conjugates are adversely affected by the presence of sodium azide, the use of which shouldtherefore be avoided. 5) The addition of Tween 20 (0.05 – 0.1%) to the conjugate diluent buffer is recommended to prevent nonspecificbinding in EIA. It may also be advantageous in other assay systems.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C.
  • バッファー
    pH: 7.20
    Preservative: 0.05% Proclin
    Constituents: 1% BSA, PBS, 10% Stabilzyme
  • Concentration information loading...
  • 精製度
    IgG fraction
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 軽鎖の種類
    unknown
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab9007 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-Fr 1/25 - 1/50.
ELISA Use at an assay dependent dilution. To give an A450nm = 1.0 using wells coated with human serum at 1/2000 dilution and TMB as a substrate: 1/6400 – 1/12800
WB Use at an assay dependent dilution. See Abreview

ターゲット情報

  • 関連性
    All five immunoglobulin classes share the same basic four polypeptide chain structure of two heavy-chains and two light chains. There are five heavy chain types, and two light-chain types (Kappa and Lambda) both having a molecular weight of 22.5kDa. Any heavy-chain type can associate with either light-chain type, but on any immunoglobulin molecule both light-chains are of the same type. Kappa and Lambda consist of a variable region and a constant region and can easily be differentiated by the antigenic properties of the constant region. The ratio of Kappa to Lambda is 70:30 , the vast majority of which is bound to heavy-chain in immunoglobulin. In normal individuals low levels of free light-chain arepresent in serum (kappa, 1.6-15.2 mg/L; Lambda, 0.4-4.2mg/L), with the occurrence of multiple myeloma or other B-cell malignancies these levels can be greatly elevated and can be found at high levels in the urine (Bence-Jones proteins).
  • 細胞内局在
    Cytoplasmic
  • 参照データベース
  • 別名
    • Bence Jones protein antibody
    • BJP antibody
    • Constant region of lambda light chains antibody
    • Ig lambda chain C regions antibody
    • ig lambda-6 chain C region antibody
    • IGLC 1 antibody
    • IGLC 2 antibody
    • IGLC 3 antibody
    • IGLC antibody
    • IGLC1 antibody
    • IGLC2 antibody
    • IGLC3 antibody
    • IGLC6 antibody
    • IGLV antibody
    • Immunoglobulin lambda constant 1 antibody
    • Immunoglobulin lambda constant regin 1 antibody
    • immunoglobulin lambda gene cluster antibody
    • Immunoglobulin lambda locus antibody
    • Immunoglobulin lambda variable cluster antibody
    • Immunoglobulin: lambda light chain antibody
    • Mcg marker antibody
    • Paraprotein antibody
    see all

画像

  • All lanes : Anti-Lambda Light chain antibody (HRP) (ab9007) at 1/500 dilution

    Lane 1 : Reduced samples (Conditioned medium from HEK293 expressing recombinant Lambda light chain).
    Lane 2 : Non-reduced samples (Conditioned medium from HEK293 expressing recombinant Lambda light chain).


    Observed band size : 25,170 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 30 kDa (possible non-specific binding).

    This image is courtesy of an anonymous Abreview

    See Abreview

参考文献

ab9007 has not yet been referenced specifically in any publications.

レビューと Q&A

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
ELISA
Sample
Human Recombinant protein (Human antibody expressed in HEK293 cells)
Specification
Human antibody expressed in HEK293 cells
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 22°C
Type
Direct
Username

Abcam user community

Verified customer

投稿 Feb 24 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Recombinant protein (Conditioned medium from HEK293 expressing rec)
Loading amount
0.05 µg
Specification
Conditioned medium from HEK293 expressing rec
Gel Running Conditions
Reduced Denaturing (9%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 22°C
Username

Abcam user community

Verified customer

投稿 Jan 27 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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