Anti-Lambda Light chain 抗体 [HP-6054] (ab49650)

製品の概要

  • 製品名Anti-Lambda Light chain antibody [HP-6054]
    Lambda Light chain 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [HP-6054] to Lambda Light chain
  • 特異性This antibody is specific to lambda immunoglobulin light chain (free or bound to heavy chain isotypes).
  • アプリケーション適用あり: WB, IHC-Fr, Flow Cyt, IHC-Pmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Purified human IgG myeloma proteins covalently coupled to polyaminostyrene micro beads.

  • ポジティブ・コントロール
    • Immunohistochemistry: Tonsil. Western Blotting: Tonsil.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーPreservative: 15mM Sodium Azide
    Constituents: Ascites
  • Concentration information loading...
  • 精製度Ascites
  • ポリ/モノモノクローナル
  • クローン名HP-6054
  • ミエローマP3-NS1/1-Ag4-1
  • アイソタイプIgG2a
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab49650 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/50 - 1/100. Predicted molecular weight: 11 kDa.
IHC-Fr 1/200 - 1/300.
Flow Cyt Use 1µg for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
IHC-P 1/1000.

ターゲット情報

  • 関連性All five immunoglobulin classes share the same basic four polypeptide chain structure of two heavy-chains and two light chains. There are five heavy chain types, and two light-chain types (Kappa and Lambda) both having a molecular weight of 22.5kDa. Any heavy-chain type can associate with either light-chain type, but on any immunoglobulin molecule both light-chains are of the same type. Kappa and Lambda consist of a variable region and a constant region and can easily be differentiated by the antigenic properties of the constant region. The ratio of Kappa to Lambda is 70:30 , the vast majority of which is bound to heavy-chain in immunoglobulin. In normal individuals low levels of free light-chain arepresent in serum (kappa, 1.6-15.2 mg/L; Lambda, 0.4-4.2mg/L), with the occurrence of multiple myeloma or other B-cell malignancies these levels can be greatly elevated and can be found at high levels in the urine (Bence-Jones proteins).
  • 細胞内局在Cytoplasmic
  • 参照データベース
  • 別名
    • Bence Jones protein antibody
    • BJP antibody
    • Constant region of lambda light chains antibody
    • Ig lambda chain C regions antibody
    • ig lambda-6 chain C region antibody
    • IGLC 1 antibody
    • IGLC 2 antibody
    • IGLC 3 antibody
    • IGLC antibody
    • IGLC1 antibody
    • IGLC2 antibody
    • IGLC3 antibody
    • IGLC6 antibody
    • IGLV antibody
    • Immunoglobulin lambda constant 1 antibody
    • Immunoglobulin lambda constant regin 1 antibody
    • immunoglobulin lambda gene cluster antibody
    • Immunoglobulin lambda locus antibody
    • Immunoglobulin lambda variable cluster antibody
    • Immunoglobulin: lambda light chain antibody
    • Mcg marker antibody
    • Paraprotein antibody
    see all

Anti-Lambda Light chain antibody [HP-6054] 画像

  • IHC image of ab49650 staining in human tonsil formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab49650, 1/1000 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
  • Overlay histogram showing Ramos cells stained with ab49650 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab49650, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Ramos cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-Lambda Light chain antibody [HP-6054] (ab49650) 使用論文

ab49650 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab49650.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"