Anti-Lambda Free Light Chain 抗体 [3D12] (ab1944)

製品の概要

  • 製品名
    Anti-Lambda Free Light Chain antibody [3D12]
    Lambda Free Light Chain 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [3D12] to Lambda Free Light Chain
  • 特異性
    Specific for independent constantly expressed epitope of lambda chain. Reacts with monomer and dimer forms of free lambda chains but not with lambda chain-bearing immunoglobulins.
  • アプリケーション
    適用あり: WB, ELISA, Other, IHC-Pmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Mixture of light chains of human immunoglobulin.

  • ポジティブ・コントロール
    • This antibody gave a positive result in IHC in the following FFPE tissue: Human normal tonsil.
  • 特記事項
    Concentration varies from lot to lot and can be provided on request.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    Preservative: 0.1% Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • 精製度
    Protein A purified
  • 特記事項(精製)
    Purity tested by electrophoresis.
  • ポリ/モノ
    モノクローナル
  • クローン名
    3D12
  • ミエローマ
    Sp2/0
  • アイソタイプ
    IgG2a
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab1944 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use at an assay dependent concentration. Predicted molecular weight: 24 kDa.
ELISA Use at an assay dependent concentration.
AP Use at an assay dependent concentration.
Other Use at an assay dependent concentration.
IHC-P Use a concentration of 5 µg/ml.

ターゲット情報

  • 関連性
    standard

Anti-Lambda Free Light Chain antibody [3D12] 画像

  • IHC image of Lambda Free Light Chain staining in Human normal tonsil formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with abX1944, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-Lambda Free Light Chain antibody [3D12] (ab1944) 使用論文

ab1944 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application
Flow Cytometry
Sample
Pig Cell (blood cells)
Permeabilization
No
Gating Strategy
Lymphoid gate only
Specification
blood cells
Preparation
Cell harvesting/tissue preparation method: hypotonic lysis of erythrocytes
Sample buffer: phosphate-buffered saline containing 0.1% sodium azide and 0.2% gelatin from Cold Water Fish Skin
Fixation
None
Username

Dr. Marek Sinkora

Verified customer

投稿 Jun 20 2016

Thank you for your call yesterday and for your patience while I have been in touch with the lab regarding your enquiry.

The protocolused for Western blotting was according to Towbin et al. in: PNAS, September 1, 1979, vol. 76, no. 9, 4350-435...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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