製品の概要

  • 製品名Anti-Lactoferrin antibody (Biotin)
    Lactoferrin 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Lactoferrin (Biotin)
  • 標識Biotin
  • 特異性Based on immunoelectrophoresis, ab25811 reacts with the human Lactoferrin found in human milk. No antibody was detected against other milk or serum proteins
  • アプリケーション適用あり: Sandwich ELISA, ELISA, WB, Flow Cyt, ICC/IFmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Full length native protein, purified from colostrum (Human)

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーPreservative: 0.05% Sodium Azide
    Constituents: 15mg/ml BSA, 0.25M Sodium chloride, 0.01M Sodium phosphate. pH 7.6
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 特記事項(精製)Isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab25811 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use a concentration of 0.5 µg/ml. Can be paired for Sandwich ELISA with Mouse monoclonal [2B8] to Lactoferrin (ab10110). For sandwich ELISA, use this antibody as Detection at 0.5µg/ml with ab10110 as Capture.
ELISA 1/20000 - 1/400000.
WB 1/20000 - 1/400000. Predicted molecular weight: 79 kDa.
Flow Cyt 1/200 - 1/1000.

ab200208 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

ICC/IF Use at an assay dependent concentration.

ターゲット情報

  • 機能Transferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate.
    Lactotransferrin has antimicrobial activity which depends on the extracellular cation concentration.
    Lactoferroxins A, B and C have opioid antagonist activity. Lactoferroxin A shows preference for mu-receptors, while lactoferroxin B and C have somewhat higher degrees of preference for kappa-receptors than for mu-receptors.
    The lactotransferrin transferrin-like domain 1 functions as a serine protease of the peptidase S60 family that cuts arginine rich regions. This function contributes to the antimicrobial activity.
  • 配列類似性Belongs to the transferrin family.
    Contains 2 transferrin-like domains.
  • 細胞内局在Secreted.
  • Information by UniProt
  • 参照データベース
  • 別名
    • GIG12 antibody
    • Growth inhibiting protein 12 antibody
    • HLF2 antibody
    • Kaliocin 1 antibody
    • Lactoferrin antibody
    • Lactoferroxin-C antibody
    • Lactotransferrin antibody
    • LF antibody
    • LTF antibody
    • Neutrophil lactoferrin antibody
    • Talalactoferrin antibody
    • TRFL_HUMAN antibody
    see all

Anti-Lactoferrin antibody (Biotin) 画像

プロトコール

Anti-Lactoferrin antibody (Biotin) (ab25811) 使用論文

This product has been referenced in:
  • Mohan R  et al. Clinical validation of integrated nucleic Acid and protein detection on an electrochemical biosensor array for urinary tract infection diagnosis. PLoS One 6:e26846 (2011). ELISA ; Human . Read more (PubMed: 22066011) »
  • Pan Y  et al. Electrochemical immunosensor detection of urinary lactoferrin in clinical samples for urinary tract infection diagnosis. Biosens Bioelectron 26:649-54 (2010). ELISA ; Human . Read more (PubMed: 20667707) »

See all 2 Publications for this product

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Ab25811 is already conjugated to biotin; there is no need to use a secondary to detect it. You can either use a Streptavidin or Avidin enzymatic (HRP) complex to react with the biotynilated antibody. There is a product...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"