The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/200 - 1/2000.
1/1000 - 1/30000. Predicted molecular weight: 78 kDa. Colorimetric: Use 1/1000-1/10000 dilution; Chemiluminescent: Use 1/1000-1/30000 dilution.
1/100 - 1/30000. Coating: Use 1/100-1/500 dilution; Primary: Use 1/1000-1/30000 dilution.
1/200 - 1/2000.
Transferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate. Lactotransferrin has antimicrobial activity which depends on the extracellular cation concentration. Lactoferroxins A, B and C have opioid antagonist activity. Lactoferroxin A shows preference for mu-receptors, while lactoferroxin B and C have somewhat higher degrees of preference for kappa-receptors than for mu-receptors. The lactotransferrin transferrin-like domain 1 functions as a serine protease of the peptidase S60 family that cuts arginine rich regions. This function contributes to the antimicrobial activity.
Belongs to the transferrin family. Contains 2 transferrin-like domains.
Western blot - Anti-Lactoferrin antibody (ab112968)This image is courtesy of an Abreview submitted by Federico Benetti.
All lanes : Anti-Lactoferrin antibody (ab112968) at 1/1000 dilution
Lane 1 : Resuspended cow lactoferrin powder at 2 µg Lane 2 : Resuspended cow lactoferrin powder at 1 µg Lane 3 : Resuspended cow lactoferrin powder at 0.5 µg Lane 4 : Resuspended cow lactoferrin powder at 0.1 µg Lane 5 : Resuspended cow lactoferrin powder at 0.05 µg Lane 6 : Resuspended cow lactoferrin powder at 0.01 µg
Secondary All lanes : Rabbit Anti-Goat IgG H&L (HRP) (ab6741) at 1/5000 dilution