製品の概要

  • 製品名Anti-Lactoferrin antibody
    Lactoferrin 一次抗体 製品一覧
  • 製品の詳細
    Goat polyclonal to Lactoferrin
  • アプリケーション適用あり: IHC-P, WB, ELISA, ICCmore details
  • 種交差性
    交差種: Cow
  • 免疫原

    Immunogen is purified lactoferrin of bovine milk origin

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: 0.09% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 特記事項(精製)Antiserum was solid phase adsorbed to ensure specificity. ab112968 was isolated by affinity chromatography using antigen coupled to agarose beads.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab112968 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/200 - 1/2000.
WB 1/1000 - 1/30000. Predicted molecular weight: 78 kDa. Colorimetric: Use 1/1000-1/10000 dilution; Chemiluminescent: Use 1/1000-1/30000 dilution.
ELISA 1/100 - 1/30000. Coating: Use 1/100-1/500 dilution; Primary: Use 1/1000-1/30000 dilution.
ICC 1/200 - 1/2000.

ターゲット情報

  • 機能Transferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate.
    Lactotransferrin has antimicrobial activity which depends on the extracellular cation concentration.
    Lactoferroxins A, B and C have opioid antagonist activity. Lactoferroxin A shows preference for mu-receptors, while lactoferroxin B and C have somewhat higher degrees of preference for kappa-receptors than for mu-receptors.
    The lactotransferrin transferrin-like domain 1 functions as a serine protease of the peptidase S60 family that cuts arginine rich regions. This function contributes to the antimicrobial activity.
  • 配列類似性Belongs to the transferrin family.
    Contains 2 transferrin-like domains.
  • 細胞内局在Secreted.
  • Information by UniProt
  • 参照データベース
  • 別名
    • GIG12 antibody
    • Growth inhibiting protein 12 antibody
    • HLF2 antibody
    • Kaliocin 1 antibody
    • Lactoferrin antibody
    • Lactoferroxin-C antibody
    • Lactotransferrin antibody
    • LF antibody
    • LTF antibody
    • Neutrophil lactoferrin antibody
    • Talalactoferrin antibody
    • TRFL_HUMAN antibody
    see all

Anti-Lactoferrin antibody 画像

  • All lanes : Anti-Lactoferrin antibody (ab112968) at 1/1000 dilution

    Lane 1 : Resuspended cow lactoferrin powder at 2 µg
    Lane 2 : Resuspended cow lactoferrin powder at 1 µg
    Lane 3 : Resuspended cow lactoferrin powder at 0.5 µg
    Lane 4 : Resuspended cow lactoferrin powder at 0.1 µg
    Lane 5 : Resuspended cow lactoferrin powder at 0.05 µg
    Lane 6 : Resuspended cow lactoferrin powder at 0.01 µg

    Secondary
    Rabbit Anti-Goat IgG H&L (HRP) (ab6741) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 78 kDa
    Observed band size : 80 kDa (why is the actual band size different from the predicted?)


    Exposure time : 1 minute

    This image is courtesy of an Abreview submitted by Federico Benetti.

    Blocked with 3% BSA for 1 hour at 20°C.

    Incubated with the primary antibody for 16 hours at 4°C in blocking buffer.

    Membrane - Nitrocellulose.

    See Abreview

Anti-Lactoferrin antibody (ab112968) 使用論文

ab112968 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Cow Purified protein (Resuspended cow lactoferrin powder)
Gel Running Conditions Reduced Denaturing (4% stacking 10% resolving)
Loading amount 2 µg
Specification Resuspended cow lactoferrin powder
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
Username

Dr. Federico Benetti

Verified customer

投稿 May 02 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"