Anti-Lactate Dehydrogenase C 抗体 [EP1746Y] (ab52747)

製品の概要

  • 製品名Anti-Lactate Dehydrogenase C antibody [EP1746Y]
    Lactate Dehydrogenase C 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EP1746Y] to Lactate Dehydrogenase C
  • 特異性This antibody reacts with Lactate Dehydrogenase C. The immunogen sequence is 100% identical to Lactate Dehydrogenase C, but there is the possibility of cross-reactivity for lactate dehydrogenase A and lactate dehydrogenase B at 69% and 82%, respectively.
  • アプリケーション適用あり: WB, Flow Cyt, IHC-Pmore details
    適用なし: ICC or IP
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Lactate Dehydrogenase C aa 300 to the C-terminus (C terminal).
    Database link: P07864

  • ポジティブ・コントロール
    • WB: Human testis lysate. IHC-P: Human testis carcinoma tissue.
  • 特記事項

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab52747 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/10000 - 1/20000. Detects a band of approximately 31 kDa (predicted molecular weight: 36 kDa).
Flow Cyt 1/50.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IHC-P Use at an assay dependent concentration.
  • 追加情報Is unsuitable for ICC or IP.
  • ターゲット情報

    • 機能Possible role in sperm motility.
    • パスウェイFermentation; pyruvate fermentation to lactate; (S)-lactate from pyruvate: step 1/1.
    • 配列類似性Belongs to the LDH/MDH superfamily. LDH family.
    • 細胞内局在Cytoplasm.
    • Information by UniProt
    • 参照データベース
    • 別名
      • Cancer/testis antigen 32 antibody
      • CT32 antibody
      • EC 1.1.1.27 antibody
      • L lactate dehydrogenase C chain antibody
      • L-lactate dehydrogenase C chain antibody
      • Lactate dehydrogenase c variant 1 antibody
      • Lactate dehydrogenase c variant 3 antibody
      • Lactate dehydrogenase c variant 4 antibody
      • Lactate dehydrogenase C4 antibody
      • LDH C antibody
      • LDH testis subunit antibody
      • LDH X antibody
      • LDH-C antibody
      • LDH-X antibody
      • LDH3 antibody
      • ldhc antibody
      • LDHC_HUMAN antibody
      • LDHX antibody
      • MGC111073 antibody
      see all

    Anti-Lactate Dehydrogenase C antibody [EP1746Y] 画像

    • Anti-Lactate Dehydrogenase C antibody [EP1746Y] (ab52747) at 1/20000 dilution + Human testis lysate at 10 µg

      Secondary
      Goat anti-rabbit HRP labeled at 1/2000 dilution

      Predicted band size : 36 kDa
      Observed band size : 31 kDa (why is the actual band size different from the predicted?)
    • Immunohistochemical staining of paraffin-embedded human testis carcinoma using ab52747 at a 1/50 dilution.

    Anti-Lactate Dehydrogenase C antibody [EP1746Y] (ab52747) 使用論文

    This product has been referenced in:
    • Rolland AD  et al. Identification of genital tract markers in the human seminal plasma using an integrative genomics approach. Hum Reprod 28:199-209 (2013). Read more (PubMed: 23024119) »
    • Wyns C  et al. Long-term spermatogonial survival in cryopreserved and xenografted immature human testicular tissue. Hum Reprod 23:2402-14 (2008). IHC-P ; Human . Read more (PubMed: 18664476) »

    See all 2 Publications for this product

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