Anti-Lactate Dehydrogenase B 抗体 [10E6AA9] (ab112996)

製品の概要

  • 製品名Anti-Lactate Dehydrogenase B antibody [10E6AA9]
    Lactate Dehydrogenase B 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [10E6AA9] to Lactate Dehydrogenase B
  • 特異性Shotgun immunization of human HeLa cell lysates into mice. Targets were determined by mass spectrometry and validated by WB, ICC, ELISA pair and other techniques.
  • アプリケーション適用あり: ICC/IF, IP, In-Cell ELISA, Flow Cytmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Hela cell cytosol fraction

  • ポジティブ・コントロール
    • HeLa cells and cell lysate.
  • 特記事項

    Product was previously marketed under the MitoSciences sub-brand.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab112996 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 2 µg/ml.
IP Use at an assay dependent concentration.
In-Cell ELISA Use a concentration of 2 µg/ml.
Flow Cyt Use a concentration of 1 - 2 µg/ml. Triton permeabilization required.



ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • パスウェイFermentation; pyruvate fermentation to lactate; (S)-lactate from pyruvate: step 1/1.
  • 関連疾患Note=Defects in LDHB result in deficiency of lactate dehydrogenase, a condition with no clear symptomatic consequences. Lactate dehydrogenase deficiency can probably be considered a non-disease.
  • 配列類似性Belongs to the LDH/MDH superfamily. LDH family.
  • 細胞内局在Cytoplasm.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Epididymis secretory protein Li 281 antibody
    • HEL S 281 antibody
    • L lactate dehydrogenase B chain antibody
    • L-lactate dehydrogenase B chain antibody
    • Lactate dehydrogenase H chain antibody
    • LDH B antibody
    • LDH H antibody
    • LDH heart subunit antibody
    • LDH-B antibody
    • LDH-H antibody
    • LDHB antibody
    • LDHB_HUMAN antibody
    • LDHBD antibody
    • LDHH antibody
    • Renal carcinoma antigen NY REN 46 antibody
    • Renal carcinoma antigen NY-REN-46 antibody
    • TRG-5 antibody
    • TRG5 antibody
    see all

Anti-Lactate Dehydrogenase B antibody [10E6AA9] 画像

  • ab112996 at 2ug/ml staining Lactate Dehydrogenase B in HeLa cells by Immunocytochemistry (4% paraformaldehyde fixed and 0.1% Triton X-100 permeabilized) followed by Alexa Fluor® 594 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1 hour (red). The target protein locates mainly to the cytoplasm.
  • ab112996 at 1ug/ml staining Lactate Dehydrogenase B in Triton X-100 permeabilized HeLa cells by flow cytometry (blue). Isotype control antibody (red).
  • Isolation of LDHB using ab112996 by immunoprecipitation.
    The identity of the protein was confirmed by mass spectrometry. The gel was stained with colloidal Coomassie blue G.

Anti-Lactate Dehydrogenase B antibody [10E6AA9] (ab112996) 使用論文

ab112996 has not yet been referenced specifically in any publications.

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