Anti-Lactate Dehydrogenase 抗体 (Biotin) (ab7638)

製品の概要

  • 製品名Anti-Lactate Dehydrogenase antibody (Biotin)
    Lactate Dehydrogenase 一次抗体 製品一覧
  • 製品の詳細
    Goat polyclonal to Lactate Dehydrogenase (Biotin)
  • 標識Biotin
  • アプリケーション適用あり: ELISA, Immunomicroscopy, WB, Dot blotmore details
  • 種交差性
    交差種: Mouse, Rabbit, Human, Chinese Hamster
    交差が予測される動物種: a wide range of other species
  • 免疫原

    Full length native Lactate Dehydrogenase (purified).

  • ポジティブ・コントロール
    • purified enzyme from rabbit muscle(Worthington Biochemicals)
  • 特記事項

    Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per goat IgG molecule.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab7638 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA
Immunomicroscopy
WB
Dot blot
  • 追加情報Dot: Use at an assay dependent dilution.
    ELISA: 1/4000 - 1/20000.
    IM: Use at an assay dependent dilution.
    WB: Use at an assay dependent dilution. Please note: The non-conjugated version of this antibody (ab2101) has been used in WB in the reference Zaman K et al (protocol available on the datasheet for ab2101).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • パスウェイFermentation; pyruvate fermentation to lactate; (S)-lactate from pyruvate: step 1/1.
    • 関連疾患Defects in LDHA are the cause of glycogen storage disease type 11 (GSD11) [MIM:612933]. A metabolic disorder that results in exertional myoglobinuria, pain, cramps and easy fatigue.
    • 配列類似性Belongs to the LDH/MDH superfamily. LDH family.
    • 翻訳後修飾ISGylated.
    • 細胞内局在Cytoplasm.
    • Information by UniProt
    • 参照データベース
    • 別名
      • Cell proliferation-inducing gene 19 protein antibody
      • GSD11 antibody
      • L lactate dehydrogenase B chain antibody
      • L-lactate dehydrogenase A chain antibody
      • Lactate dehydrogenase A antibody
      • Lactate dehydrogenase B antibody
      • Lactate dehydrogenase H chain antibody
      • Lactate dehydrogenase M antibody
      • LDH A antibody
      • LDH B antibody
      • LDH H antibody
      • LDH heart subunit antibody
      • LDH M antibody
      • LDH muscle subunit antibody
      • LDH-A antibody
      • LDH-M antibody
      • LDH1 antibody
      • ldha antibody
      • LDHA_HUMAN antibody
      • LDHBD antibody
      • LDHM antibody
      • MS1111 antibody
      • PIG19 antibody
      • Proliferation inducing gene 19 antibody
      • Renal carcinoma antigen NY REN 46 antibody
      • Renal carcinoma antigen NY-REN-59 antibody
      • TRG 5 antibody
      • TRG5 antibody
      see all

    Anti-Lactate Dehydrogenase antibody (Biotin) (ab7638) 使用論文

    This product has been referenced in:
    • Rudyk O  et al. Protein kinase G oxidation is a major cause of injury during sepsis. Proc Natl Acad Sci U S A 110:9909-13 (2013). Mouse . Read more (PubMed: 23716652) »
    • Jerde TJ  et al. STRETCH INDUCTION OF CYCLOOXYGENASE-2 EXPRESSION IN HUMAN UROTHELIAL CELLS IS CALCIUM AND PROTEIN KINASE C ZETA-DEPENDENT. Mol Pharmacol : (2007). WB ; Human . Read more (PubMed: 17925460) »

    See all 3 Publications for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application ELISA
    Sample Chinese Hamster Cell (Ovary)
    Specification Ovary
    Type Direct
    Username

    Abcam user community

    Verified customer

    投稿 Apr 11 2012

    I am very pleased to hear the customer would like to accept our offer and test ab7638 in Chinese hamster.

    DISCOUNT CODE: #####
    Expiration date: ######

    This code will give you 1 free primary antibodybefore the expiration date....

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    Thank you for your enquiry.

    I am sorry to confirm that as far as we are aware, ab7638 Lactate Dehydrogenase antibody (Biotin)] has never been tested with samples from Chinese hamster. All tested and guaranteed species cross-reactivity inform...

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    Unfortunately, this information is not known.

    The unconjugated form (ab2101) was raised against rabbit LDH. This is expected to react with other species, although this has not been tested yet.

    ab7638 is linked to biotin. For Western blotting procedures such as this we would recommend ab2101 followed by a secondary against goat linked to HRP (such as ab7125).

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"