Anti-Lactate Dehydrogenase 抗体 [AF14A11] (ab85326)

製品の概要

  • 製品名Anti-Lactate Dehydrogenase antibody [AF14A11]
    Lactate Dehydrogenase 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [AF14A11] to Lactate Dehydrogenase
  • アプリケーション適用あり: Flow Cyt, WB, IP, ELISAmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    His tagged recombinant Human Lactate Dehydrogenase protein purified from E.coli.

  • ポジティブ・コントロール
    • HeLa, MCF7, HepG2, 293T, SK-N-MC, RD and IMR32 cell lysates.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab85326 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt 1/100. ab170192-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
WB 1/2000 - 1/10000. Detects a band of approximately 30 kDa.
IP Use at an assay dependent concentration. Use 0.5µg
ELISA 1/2000 - 1/10000.

ターゲット情報

  • パスウェイFermentation; pyruvate fermentation to lactate; (S)-lactate from pyruvate: step 1/1.
  • 関連疾患Defects in LDHA are the cause of glycogen storage disease type 11 (GSD11) [MIM:612933]. A metabolic disorder that results in exertional myoglobinuria, pain, cramps and easy fatigue.
  • 配列類似性Belongs to the LDH/MDH superfamily. LDH family.
  • 翻訳後修飾ISGylated.
  • 細胞内局在Cytoplasm.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Cell proliferation-inducing gene 19 protein antibody
    • GSD11 antibody
    • L lactate dehydrogenase B chain antibody
    • L-lactate dehydrogenase A chain antibody
    • Lactate dehydrogenase A antibody
    • Lactate dehydrogenase B antibody
    • Lactate dehydrogenase H chain antibody
    • Lactate dehydrogenase M antibody
    • LDH A antibody
    • LDH B antibody
    • LDH H antibody
    • LDH heart subunit antibody
    • LDH M antibody
    • LDH muscle subunit antibody
    • LDH-A antibody
    • LDH-M antibody
    • LDH1 antibody
    • ldha antibody
    • LDHA_HUMAN antibody
    • LDHBD antibody
    • LDHM antibody
    • MS1111 antibody
    • PIG19 antibody
    • Proliferation inducing gene 19 antibody
    • Renal carcinoma antigen NY REN 46 antibody
    • Renal carcinoma antigen NY-REN-59 antibody
    • TRG 5 antibody
    • TRG5 antibody
    see all

Anti-Lactate Dehydrogenase antibody [AF14A11] 画像

  • All lanes : Anti-Lactate Dehydrogenase antibody [AF14A11] (ab85326) at 1/2000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : MCF7 cell lysate
    Lane 3 : HepG2 cell lysate
    Lane 4 : 293T cell lysate
    Lane 5 : SK-N-MC cell lysate
    Lane 6 : RD cell lysate
    Lane 7 : IMR32 cell lysate


    Observed band size : 30 kDa (why is the actual band size different from the predicted?)
  • Overlay histogram showing HeLa cells stained with ab85326 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab85326, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-Lactate Dehydrogenase antibody [AF14A11] (ab85326) 使用論文

This product has been referenced in:
  • Tseng CY & Firestein BL The Role of PSD-95 and Cypin in Morphological Changes in Dendrites Following Sublethal NMDA Exposure. J Neurosci 31:15468-80 (2011). WB ; Rat . Read more (PubMed: 22031893) »

See 1 Publication for this product

Product Wall

ab85326 was raised against recombinant human lactate dehydrogenase, not a synthetic peptide, and the antibody has not been epitope mapped.
The antibody is specific to LDHA, which is found predominantly in muscle tissue.
We have tested the cross...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HCT116)
Loading amount 10 µg
Specification HCT116
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5%
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投稿 Oct 13 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"