製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to L3MBTL2
  • アプリケーション
    適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Pig
  • 免疫原

    Synthetic peptide corresponding to a region of human L3MBTL2 (NP_001003689)

  • ポジティブ・コントロール
    • HeLa cell lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab80489 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 69 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/312500.

ターゲット情報

  • 機能
    Putative Polycomb group (PcG) protein. PcG proteins maintain the transcriptionally repressive state of genes, probably via a modification of chromatin, rendering it heritably changed in its expressibility. Its association with a chromatin-remodeling complex suggests that it may contribute to prevent expression of genes that trigger the cell into mitosis. Binds to monomethylated and dimethylated 'Lys-20' on histone H4. Binds histone H3 peptides that are monomethylated or dimethylated on 'Lys-4', 'Lys-9' or 'Lys-27'.
  • 配列類似性
    Contains 1 FCS-type zinc finger.
    Contains 4 MBT repeats.
  • 細胞内局在
    Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 3bt like 2 protein antibody
    • 3bt like protein antibody
    • DKFZP761I141 antibody
    • H-l(3)mbt-like protein 2 antibody
    • H-l(3)mbt-like protein antibody
    • Hl antibody
    • Hl(3)mbt like protein antibody
    • Hl(3)mbtl antibody
    • L antibody
    • l(3)mbt like 2 (Drosophila) antibody
    • l(3)mbt like 2 antibody
    • L(3)mbt-like 2 protein antibody
    • L(3)mbt-like protein 2 antibody
    • L3MBTL2 antibody
    • Lethal antibody
    • Lethal(3)malignant brain tumor-like 2 protein antibody
    • Lethal(3)malignant brain tumor-like protein 2 antibody
    • LMBL2_HUMAN antibody
    see all

画像

  • Anti-L3MBTL2 antibody (ab80489) at 1 µg/ml + HeLa cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 69 kDa
    Observed band size : 69 kDa
    Additional bands at : 27 kDa,40 kDa. We are unsure as to the identity of these extra bands.

プロトコール

参考文献

ab80489 has not yet been referenced specifically in any publications.

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