Anti-L3MBTL2 抗体 (ab101272)
Key features and details
- Rabbit polyclonal to L3MBTL2
- Suitable for: IHC-P, IP
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-L3MBTL2 antibody
L3MBTL2 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to L3MBTL2 -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, IPmore details -
種交差性
交差種: Human
交差が予測される動物種: Cow, Chimpanzee, Gorilla, Orangutan -
免疫原
Synthetic peptide corresponding to a region between residue 655 and 705 of Human L3MBTL2 (NP_113676.2).
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ポジティブ・コントロール
- HeLa whole cell lysate
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
ab101272 was affinity purified using an epitope specific to L3MBTL2 immobilized on solid support. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab101272の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P |
1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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IP |
Use at 2-5 µg/mg of lysate.
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特記事項 |
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IHC-P
1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
IP
Use at 2-5 µg/mg of lysate. |
ターゲット情報
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機能
Putative Polycomb group (PcG) protein. PcG proteins maintain the transcriptionally repressive state of genes, probably via a modification of chromatin, rendering it heritably changed in its expressibility. Its association with a chromatin-remodeling complex suggests that it may contribute to prevent expression of genes that trigger the cell into mitosis. Binds to monomethylated and dimethylated 'Lys-20' on histone H4. Binds histone H3 peptides that are monomethylated or dimethylated on 'Lys-4', 'Lys-9' or 'Lys-27'. -
配列類似性
Contains 1 FCS-type zinc finger.
Contains 4 MBT repeats. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 513297 Cow
- Entrez Gene: 83746 Human
- Entrez Gene: 100173412 Orangutan
- Omim: 611865 Human
- SwissProt: Q1JQD9 Cow
- SwissProt: Q969R5 Human
- SwissProt: Q5R737 Orangutan
- Unigene: 517641 Human
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別名
- 3bt like 2 protein antibody
- 3bt like protein antibody
- DKFZP761I141 antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling L3MBTL2 with ab101272 at 1/1000 (1µg/ml).
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Detection of L3MBTL2 in Immunoprecipitates of HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) using ab101272 at 3 µg/mg lysate for IP (Lane 1). An anti-L3MBTL2 antibody which recognizes an upstream epitope was used in Lane 2, and also for the subsequent WB detection at 1 µg/ml. Lane 3 represents control IgG. Detection: Chemiluminescence with an exposure time of 30 seconds.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab101272 は論文での使用が確認できていません。