製品の概要

  • 製品名
    L-Glutamate
  • 製品の詳細
    Excitatory neurotransmitter
  • 生理活性の詳細
    Major excitatory neurotransmitter. Acts at glutamate receptors. Active in vivo.
  • 精製度
    > 99%

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab120049 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Functional Studies Use at an assay dependent concentration.

画像

  • ab55051 staining GABA B receptor 1 in SK-N-SH cells treated with L-Glutamate (ab120049), by ICC/IF. Internalization of GABA B receptor 1 correlates with increased concentration of L-Glutamate, as described in literature.
    The cells were incubated at 37°C for 30 minutes in media containing different concentrations of ab120049 (L-Glutamate) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab55051 (1 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-mouse polyclonal antibody (ab96879) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.

参考文献

This product has been referenced in:
  • Hainsworth AH  et al. Glutamate receptor-mediated inhibition of L-glutamate efflux from cerebral cortex in vitro. Brain Res 1114:36-40 (2006). Read more (PubMed: 16904087) »
  • Kew JN & Kemp JA Ionotropic and metabotropic glutamate receptor structure and pharmacology. Psychopharmacology (Berl) 179:4-29 (2005). Read more (PubMed: 15731895) »

See 0 Publications for this product

レビューと Q&A

To prepare the 1 gram vial in 1 eq. NaOH, you'll need to solubilize each 14.7 mg of the L-glutamate in 1 mL of 100 mM NaOH (due to batch-specific molecular weight of 147.13 g/mol). So, to prepare the full vial, you'll add 68.03 mL of 100 mM NaOH (10...

Read More

The answer to this can be found on the biochemicals FAQ section of the PWS. http://www.abcam.com/index.html?pageconfig=resource&rid=14735#NaOH

If you look under the Solubilisation heading then “How do I make solutions using NaOH equi...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE, NOT FOR USE IN HUMANS"

登録